Sophisticated Instrumental Evaluation of Novel Siddha Raw Drug -Manosilai: An Modern Approach towards Drug Standardization

In Siddha system of traditional medicine minerals and animal products are used as main drugs to treat various dreadful diseases. Standardization of Siddha preparations is of utmost important task in establishing the active components of the drug responsible for its biological activity. WHO has emphasized the need to ensure quality control of Indian Medicines including Siddha formulations by using modern techniques and by applying suitable parameters and standards. Siddha practitioners use several metallic preparations to treat diseases such as cancer, urolithiasis, kidney disorders and chronic liver diseases. One such novel drug is Manosilai comprises of arsenic and Sulphur as major components. The main aim of the present study is to purify the Manosilai and to analyses the same with modern sophisticated analytical instrumentation techniques. ICPOES results of the sample manosilai before and after purification reveals the presence of Mercury, Lead, Arsenic and Cadmium along with other trace elements. FT-IR analysis report of both unpurified and purified formulation confirm the presence of biologically significant functional group with characteristic IR absorption frequencies. Further the XRD patter of samples justifies the presence of arsenic sulfide and mercury being the major component of the manosilai. SEM analysis of the sample signifies that the mean particle size of the formulation ranges from 11.21 to 19.37 μm. Hence from the results of the present investigation it was clear that the manosilai purified in accordance with traditional siddha system confirms the stability and complies with the genuinity and standards as per AYUSH guidelines.

[1]  B. D. Gupta,et al.  MEDICINAL PLANTS OF INDIA WITH ANTIDIABETIC POTENTIAL , 1993 .

[2]  M. Heinrich,et al.  Value chains of herbal medicines--research needs and key challenges in the context of ethnopharmacology. , 2012, Journal of ethnopharmacology.

[3]  C. Stadtländer Scanning Electron Microscopy and Transmission Electron Microscopy of Mollicutes : Challenges and Opportunities , 2007 .

[4]  Galib,et al.  Therapeutic potentials of metals in ancient India: A review through Charaka Samhita , 2011, Journal of Ayurveda and integrative medicine.

[5]  M. Sekar,et al.  Evaluation of dentinal tubule occlusion and depth of penetration of nano-hydroxyapatite derived from chicken eggshell powder with and without addition of sodium fluoride: An in vitro study , 2016, Journal of conservative dentistry : JCD.

[6]  R. Cohen,et al.  A Concise History of Science in India , 1976 .

[7]  M. Srivastava High-Performance Thin-Layer Chromatography (HPTLC) , 2011 .

[8]  S. Yadav,et al.  Medicinal plants of India with anti-diabetic potential. , 2002, Journal of ethnopharmacology.

[9]  A. Austin Chemical Characterization of a Gold and Mercury based Siddha Sasthric Preparation-Poorna chandrodayam , 2012 .

[10]  C. Padmalatha,et al.  Antibacterial potential of some herbo-mineral siddha preparation : An alternative medicine for enteric pathogens , 2011 .

[11]  Namakkal,et al.  FT-IR STUDIES ON THE LEAVES OF ALBIZIA , 2012 .

[12]  A. Panda,et al.  ARSENICAL COMPOUNDS IN AYURVEDA MEDICINE : A PROSPECTIVE ANALYSIS , 2012 .

[13]  A. Alomary,et al.  Determination of heavy metals (Cd, Cr, Cu, Fe, Ni, Pb, Zn) by ICP-OES and their speciation in Algerian Mediterranean Sea sediments after a five-stage sequential extraction procedure , 2007, Environmental monitoring and assessment.

[14]  H. Mantsch,et al.  Determination of protein secondary structure by Fourier transform infrared spectroscopy: a critical assessment. , 1993, Biochemistry.

[15]  John Chamberlain,et al.  The determination of refractive index spectra by fourier spectrometry , 1969 .

[16]  K. D. Vernon-Parry,et al.  Scanning Electron Microscopy , 2012, Current protocols in microbiology.