Post-translational Modifications in the Cardiovascular Proteome

The analysis of post-translational modifications is critical for understanding the regulation of protein function in the heart. These small, often charged, groups are added to a protein’s structure to modulate its activity, localization or associations. The development of proteomic technologies has greatly improved the identification and subsequent characterization of these modifications. However, due to the complex nature of the cardiovascular proteome, a particular post-translational modification may represent only a tiny fraction of the milieu. Additionally, some modifications are too labile for mass spectrometry analysis. To address these limitations, a variety of techniques and strategies have been developed to specifically target and improve the detection of these modifications. In the following chapter, we review the challenges and solutions to identifying several prominent post-translational modifications in the cardiovascular system.

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