Stationary phase mutagenesis in Bacillus subtilisis independent of genome replication

References Acknowledgements •Stationary phase mutagenesis is the process by which cells under non-growing conditions accumulate mutations in genes under selection •This type of cellular programs are associated with the formation of neoplasia in animal cells and with acquisition of antibiotic resistance and evasion of immune responses in in microbial pathogens (Galhardo, 2007) •This process have been extensively studied in Escherichia coli and indicate that stress-induced, or stationary phase mutations are generated during the processing of intermediates formed during genome replication (Galhardo, 2007; Hastings, 2007) •In Bacillus subtilis, however, several factors required for the processing of replication intermediates are not required and suggest that stationary phase mutations are produced by a different mechanism (Robleto et al, 2007) •Here we examine stationary phase mutagenesis in the absence of genome replication and showed that B. subtilis produces mutations in non-growing cells by mechanisms that do not require genomic replication. KThThT 2 3 4 5 6 * 7 8 * 9 1 0