论文信息 - Detailed Protocol for Purification of Chlamydia pneumoniae Elementary Bodies

Detailed Protocol for Purification of Chlamydia pneumoniae Elementary Bodies

ABSTRACT A detailed protocol for the growth and harvest of purified elementary bodies of Chlamydia pneumoniae is presented. This procedure utilizes a flask-to-flask passage scheme designed to achieve high bacterial titers in a short period of time.

Richardd . Miller | J. Summersgill | Sanghamitra Mukhopadhyay | Erin D. Sullivan | A. P. Clark

[1] B. Coombes,et al. Analytical Sensitivity, Reproducibility of Results, and Clinical Performance of Five PCR Assays for Detecting Chlamydia pneumoniae DNA in Peripheral Blood Mononuclear Cells , 2000, Journal of Clinical Microbiology.

[2] H. Caldwell,et al. Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis , 1981, Infection and immunity.

[3] C. Kuo,et al. Interaction of Chlamydia trachomatis organisms and HeLa 229 cells , 1976, Infection and immunity.