Pattern of bromodeoxyuridine incorporation in the advanced stages of arm regeneration in the feather star Antedon mediterranea

Abstract.The overall process of arm regeneration in the crinoid Antedon mediterranea is a typical epimorphic process (blastemal regeneration). This can be subdivided into three main phases: a repair phase, an early regenerative phase, and an advanced regenerative phase. The crucial problem of the identification of cell lineages responsible for both repair and regenerative processes has been approached by monitoring cell proliferation during the advanced regenerative phase using light-microscopic and ultrastructural immunocytochemical methods to detect the incorporation of the thymidine analogue bromodeoxyuridine (BrdU) into regenerating tissues. Various treatment protocols and BrdU incubation times have been employed and provided information not only on the sources, sites of proliferation, and recruitment times of the new cells, but also on the cell lineage involved and subsequent fate (differentiation and/or migration) of the labelled cells. Our results are consistent with the following conclusions: (1) The arm regeneration process is due to a massive intervention of active proliferating cells identifiable as migratory, morphologically undifferentiated cells (amoebocytes and coelomocytes). (2) The preferential proliferation sites of these cells are the terminal blastema, the coelomic epithelium, and the brachial nerve of both the regenerating arm and the stump, even far from the amputation. (3) The two main cell components contributing to the regenerate have different origins: the blastemal cells and all the cell lineages derived from the amoebocytes; the coelomic cells from the migratory coelomocytes, in their turn derived from proliferation of the coelomic epithelium. (4) The blastemal regeneration of Antedon is due to a combined recruitment of pluripotent elements, implying the intervention of presumptive stem cells (amoebocytes) and the transdifferentiation/dedifferentiation of differentiated elements of the coelomic epithelium.

[1]  August Reichensperger,et al.  Beiträge zur Histologie und zum Verlauf der Regeneration bei Crinoiden , 1912 .

[2]  T. Norwood,et al.  Selective elimination of proliferating cells in human diploid cell cultures by treatment with BrdU, 33258 Hoechst and visible light , 1980, Mechanisms of Ageing and Development.

[3]  H. Gratzner,et al.  Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication. , 1982, Science.

[4]  I. Wilkie Variable tensility in echinoderm collagenous tissues: A review , 1984 .

[5]  M. Carnevali,et al.  Muscle system organization in the echinoderms: II. Microscopic anatomy and functional significance of the muscle‐ligament‐skeleton system in the arm of the comatulids (Antedon mediterranea) , 1985, Journal of morphology.

[6]  M. Carnevali,et al.  Muscle system organization in the echinoderms: III. Fine structure of the contractile apparatus of the arm flexor muscles of the comatulids (Antedon mediterranea) , 1985, Journal of morphology.

[7]  R. Davidson,et al.  DNA base sequence changes and sequence specificity of bromodeoxyuridine-induced mutations in mammalian cells. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[8]  M. Daniela Candia-Carnevali,et al.  Regeneration and Morphogenesis in the Feather Star Arm , 1989 .

[9]  S. Tapscott,et al.  5-bromo-2'-deoxyuridine blocks myogenesis by extinguishing expression of MyoD1. , 1989, Science.

[10]  S. Amemiya,et al.  Regeneration in sea lilies , 1992, Nature.

[11]  M. Daniela Candia Carnevali,et al.  Mechanisms of arm regeneration in the feather star Antedon mediterranea: Healing of wound and early stages of development , 1993 .

[12]  F. Bonasoro,et al.  Mechanism of arm regeneration in Antedon mediterranea (Echinodermata, Crinoidea) , 1994 .

[13]  U. Welsch,et al.  Neural factors in crinoid arm regeneration , 1995 .

[14]  M. Daniela Candia Carnevali,et al.  Pattern of cell proliferation in the early stages of arm regeneration in the feather star Antedon mediterranea , 1995 .

[15]  M. Daniela Candia Carnevali,et al.  Tissue distribution of monoamine neurotransmitters in normal and regenerating arms of the feather star Antedon mediterranea , 1996, Cell and Tissue Research.

[16]  J. Becerra,et al.  Incorporation of bromodeoxyuridine in regenerating fin tissue of the goldfish Carassius auratus. , 1996, The Journal of experimental zoology.

[17]  U. Welsch,et al.  Arm regeneration and growth factors in crinoids , 1998 .

[18]  Candia Carnevali,et al.  Epimorphic versus morphallactic mechanism in arm regeneration of crinoids and asteroids: pattern of cell proliferation/differentiation and cell lineage , 1998 .