论文信息 - Reducing image distortions due to temperature‐related microscope stage drift

Reducing image distortions due to temperature‐related microscope stage drift

Distortions in confocal 3D image data sets were related to movements of the microscope stage that matched fluctuations in laboratory temperature. Movement was apparent in all three axes. Adding a draught‐proof enclosure, covering the stage, objective lenses and supporting structures, minimized these short‐term fluctuations. However, the stage still tracked slow changes in laboratory temperature and was sensitive to heat sources mounted on the microscope. Suggestions are made about microscope design.

J Adler | S N Pagakis | S. Pagakis | J. Adler

[1] S. Erasmus. Reduction of noise in TV rate electron microscope images by digital filtering , 1982 .

[2] K. Jacobson,et al. Single-particle tracking: applications to membrane dynamics. , 1997, Annual review of biophysics and biomolecular structure.

[3] J. Pawley,et al. Handbook of Biological Confocal Microscopy , 1990, Springer US.

[4] A. Periasamy. Methods in Cellular Imaging , 2001, Methods in Physiology.

[5] J R Nyengaard,et al. Tissue shrinkage and unbiased stereological estimation of particle number and size * , 2001, Journal of microscopy.

[6] S. Hell,et al. Focal spots of size lambda/23 open up far-field fluorescence microscopy at 33 nm axial resolution. , 2002, Physical review letters.

[7] D. Klenerman,et al. Simultaneous measurement of Ca2+ and cellular dynamics: combined scanning ion conductance and optical microscopy to study contracting cardiac myocytes. , 2001, Biophysical journal.

[8] Jason R Swedlow,et al. Measuring tubulin content in Toxoplasma gondii: A comparison of laser-scanning confocal and wide-field fluorescence microscopy , 2002, Proceedings of the National Academy of Sciences of the United States of America.