Estimation of terpenoid content and its antimicrobial property in Enicostemma litorrale

Enicostemma littorale is traditionally used in India as a stomachic, bitter tonic, carminative to reduce fever and as a tonic for appetite loss. In Indian ayurvedic medicine, E. littorale is taken in combination with other herbs, especially for diabetes. This study aims to determine the presence of terpenoid content in E. littorale both qualitatively and quantitatively. The plant sample is extracted with 5 different solvent viz. ethanol, petroleum ether, chloroform, water and acetone. The presence of terpenoid was estimated by phytochemical analysis and quantitative study using linalool reagent. Antimicrobial activity of the terpenoid extract was tested against E. coli and B. Subtilis. Formation of reddish brown color indicated the presence of terpenoid. The total amount of terpenoid present in the E. littorale sample was found to be 0.7%. Considerable diameter of zone of inhibition was observed against E. coli and B. Subtilis.