Investigations into (cid:2) B -Modulated Regulatory Pathways Governing Extracellular Virulence Determinant Production in Staphylococcus aureus

The commonly used Staphylococcus aureus laboratory strain 8325-4 bears a naturally occurring 11-bp deletion in the (cid:1) B -regulating phosphatase rsbU . We have previously published a report (M. J. Horsburgh, J. L. Aish, I. J. White, L. Shaw, J. K. Lithgow, and S. J. Foster, J. Bacteriol. 184:5457–5467, 2002) on restoring the rsbU deletion, producing a (cid:1) B -functional 8325-4 derivative, SH1000. SH1000 is pleiotropically altered in phenotype from 8325-4, displaying enhanced pigmentation, increased growth yields, and a marked decrease in secreted exoproteins. This reduction in exoprotein secretion appears to result from a sixfold reduction in agr expression. In this study we have undertaken transposon mutagenesis of SH1000 to identify components involved in the modulation of extracellular proteases and (cid:2) is a de- rivative of has had cassette tetracycline To generate a EcoRI/NotI-flanked (containing was into To generate a using was into to

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