Immunolocalization of osteonectin in avian tibial dyschondroplastic cartilage

Osteonectin is an acidic calcium‐binding protein found in cartilage, bone matrix, vascular endothelium, and areas of tissue repair. Using immunocytochemistry, osteonectin has been localized in all zones of the normal avian epiphyseal growth plate with notably high amounts in the hypertrophic zone. In the proximal portion of this zone the staining was intracellular, while in the distal calcifying portion of the hypertrophic zone staining was both intracellular and extracellular. Osteonectin was also detected in the growth plate associated with lesions of chickens with tibial dyschondroplasia (TD). Intense intracellular staining was observed in hypertrophic chondrocytes proximal to the lesion; staining was markedly diminished in the TD lesion; extracellular matrix was devoid of staining. Staining intensity was high along the peripheral edges of the lesion that were undergoing vascularization and resorption. This was the only area in the dysplastic cartilage where staining was observed in the extracellular matrix as well as intracellularly. Similar patterns were viewed in all TD lesions examined, whether they were spontaneous or induced by dietary treatments or genetic selection. © 1996 Wiley‐Liss, Inc.

[1]  D. Nie,et al.  Defect in formation of functional matrix vesicles by growth plate chondrocytes in avian tibial dyschondroplasia: Evidence of defective tissue vascularization , 1995, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[2]  C. Farquharson,et al.  Regulators of chondrocyte differentiation in tibial dyschondroplasia: an in vivo and in vitro study. , 1995, Bone.

[3]  M. Paulsson,et al.  Transglutaminase-catalyzed matrix cross-linking in differentiating cartilage: identification of osteonectin as a major glutaminyl substrate , 1995, The Journal of cell biology.

[4]  M. Pines,et al.  Osteopontin gene expression and alkaline phosphatase activity in avian tibial dyschondroplasia. , 1995, Bone.

[5]  G. Rosselot,et al.  Effect of lesion size on the metabolic activity of tibial dyschondroplastic chondrocytes. , 1994, Poultry science.

[6]  E. Sage,et al.  The biology of SPARC, a protein that modulates cell‐matrix interactions , 1994, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[7]  C. Farquharson,et al.  Avian tibial dyschondroplasia: the interaction of genetic selection and dietary 1,25-dihydroxycholecalciferol. , 1993, Avian pathology : journal of the W.V.P.A.

[8]  R. Leach,et al.  Chicken tibial dyschondroplasia: A limb mutant with two growth plates and possible defects of collagen crosslinking , 1993, Developmental dynamics : an official publication of the American Association of Anatomists.

[9]  S. Mundlos,et al.  Distribution of osteonectin mRNA and protein during human embryonic and fetal development. , 1992, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[10]  R. Timpl,et al.  Solubilization of protein BM‐40 from a basement membrane tumor with cheating agents and evidence for its identity with osteonectin and SPARC , 1987, FEBS letters.

[11]  C. Gay,et al.  Tritiated thymidine uptake in chondrocytes of chickens afflicted with tibial dyschondroplasia. , 1985, Avian diseases.

[12]  C. Gay,et al.  Activities and distribution of alkaline phosphatase and carbonic anhydrase in the tibial dyschondroplastic lesion and associated growth plate of chicks. , 1985, Avian diseases.

[13]  C. Gay,et al.  Avian tibial dyschondroplasia. I. Ultrastructure. , 1985, The American journal of pathology.

[14]  C. Gay,et al.  Avian tibial dyschondroplasia. II. Biochemical changes. , 1985, The American journal of pathology.

[15]  G. Ruth,et al.  Dyschondroplasias, including osteochondrosis, in boars between 25 and 169 days of age: histologic changes. , 1984, American journal of veterinary research.

[16]  P. Bornstein,et al.  Characterization of a novel serum albumin-binding glycoprotein secreted by endothelial cells in culture. , 1984, The Journal of biological chemistry.

[17]  H. Kleinman,et al.  Osteonectin, a bone-specific protein linking mineral to collagen , 1981, Cell.

[18]  M. Lilburn,et al.  Metabolism of abnormal cartilage cells associated with tibial dyschondroplasia. , 1980, Poultry science.

[19]  H. C. Robinson,et al.  Cartilage matrix components in chickens with tibial dyschondroplasia. , 1974, The Journal of nutrition.

[20]  M. Orth,et al.  Non-reducible collagen cross-linking in cartilage from broiler chickens with tibial dyschondroplasia. , 1994, Avian diseases.

[21]  S. Jimenez,et al.  Type X collagen in avian tibial dyschondroplasia. , 1989, Laboratory investigation; a journal of technical methods and pathology.

[22]  Glade Mj,et al.  Growth plate cartilage metabolism, morphology and biochemical composition in over- and underfed horses. , 1984 .