Mutations on Fibrinogen (γ316–322) Are Associated with Reduction in Platelet Adhesion Under Flow Conditions

Abstract: In this paper we report on studies of platelet adhesion to several fibrinogen γ chain variants under physiological flow conditions. Reduced platelet adhesion was found to patient dysfibrinogen Vlissingen and its recombinant form (deletion of γ319–320). Furthermore, substitutions of the amino acids 318, 320, or both in the recombinant fibrinogen γ chain showed a strong decrease in platelet adhesion under flow conditions in our perfusion system. Antibodies raised against peptides covering these sequences inhibited platelet adhesion completely, which suggested that the γ316–322 sequence could be involved in platelet adhesion in flowing blood.

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