Lipid bilayer thickness varies linearly with acyl chain length in fluid phosphatidylcholine vesicles.

The thickness of the lipid bilayer in vesicles made of pure phosphatidylcholines, with acyl chain lengths ranging from 10 to 24 carbons, has been determined by analysis of continuous X-ray scattering data from vesicle pellets at temperatures above the lipid phase transition temperature. Bilayer thickness was found to vary linearly with the number of carbons per acyl chain. The lines for saturated and monounsaturated acyl chains were slightly displaced but had similar slopes. For the saturated species di-12:0, di-14:0, di-16:0, and di-18:0 phosphatidylcholine the surface areas per molecule were all 65.7 to 66.5 A2, while the monounsaturated species and di-10:0 phosphatidylcholine all occupied 67.7 to 70.1 A2 per molecule.

[1]  B. de Kruijff,et al.  The preference of cholesterol for phosphatidylcholine in mixed phosphatidylcholine-phosphatidylethanolamine bilayers. , 1976, Biochimica et biophysica acta.

[2]  F A Quiocho,et al.  The radius of gyration of L-arabinose-binding protein decreases upon binding of ligand. , 1981, The Journal of biological chemistry.

[3]  L. J. Lis,et al.  Interactions between neutral phospholipid bilayer membranes. , 1982, Biophysical journal.

[4]  B. Lentz,et al.  Effect of lipid membrane structure on the adenosine 5'-triphosphate hydrolyzing activity of the calcium-stimulated adenosinetriphosphatase of sarcoplasmic reticulum. , 1981, Biochemistry.

[5]  D. Engelman,et al.  Lipid bilayer structure in the membrane of Mycoplasma laidlawii. , 1971, Journal of molecular biology.

[6]  C. D. Richards,et al.  The effect of bilayer thickness and n-alkanes on the activity of the (Ca2+ + Mg2+)-dependent ATPase of sarcoplasmic reticulum. , 1981, The Journal of biological chemistry.

[7]  T. Steitz,et al.  Yeast hexokinase in solution exhibits a large conformational change upon binding glucose or glucose 6-phosphate. , 1979, Biochemistry.

[8]  M. Caffrey,et al.  Fluorescence quenching in model membranes. 3. Relationship between calcium adenosinetriphosphatase enzyme activity and the affinity of the protein for phosphatidylcholines with different acyl chain characteristics. , 1981, Biochemistry.

[9]  F. Reiss-Husson,et al.  The Structure of the Micellar Solutions of Some Amphiphilic Compounds in Pure Water as Determined by Absolute Small-Angle X-Ray Scattering Techniques , 1964 .

[10]  G Büldt,et al.  Neutron diffraction studies on phosphatidylcholine model membranes. II. Chain conformation and segmental disorder. , 1979, Journal of molecular biology.

[11]  D. Engelman,et al.  CURRENT MODELS FOR THE STRUCTURE OF BIOLOGICAL MEMBRANES , 1969, The Journal of cell biology.

[12]  J. Nagle,et al.  Lecithin bilayers. Density measurement and molecular interactions. , 1978, Biophysical journal.

[13]  E. Evans,et al.  Thermoelasticity of large lecithin bilayer vesicles. , 1981, Biophysical journal.

[14]  G Büldt,et al.  Neutron diffraction studies on phosphatidylcholine model membranes. I. Head group conformation. , 1979, Journal of molecular biology.

[15]  D. Engelman,et al.  Bacteriorhodopsin remains dispersed in fluid phospholipid bilayers over a wide range of bilayer thicknesses. , 1983, Journal of molecular biology.

[16]  D. Engelman,et al.  X-ray diffraction studies of phase transitions in the membrane of Mycoplasma laidlawii. , 1970, Journal of molecular biology.

[17]  S B Hladky,et al.  Ion transport across thin lipid membranes: a critical discussion of mechanisms in selected systems , 1972, Quarterly Reviews of Biophysics.