Monocyte/macrophage-reactive monoclonal antibody Ki-M6 recognizes an intracytoplasmic antigen.

A monoclonal antibody, termed Ki-M6, is described, which shows a restricted reactivity to cells of the monocyte/macrophage system. On light- and electron-microscopic immunoperoxidase staining Ki-M6 recognizes monocytes and the phagocytosing compartment of macrophages residing in different tissue sites; granulocytes and the so-called immune accessories of B- and T-cell immune response as closely monocyte/macrophage related cell populations do not reveal any reactivity. This is shown by comparison with the monoclonal antibodies Ki-M4 and Ki-M1 or OKT6 recognizing immune accessory cells by immunohistochemical methods. Ki-M6 binds to a lysosomal membrane-restricted antigen of 60,000 daltons without influencing significantly lysosome-related functions as far as the chemiluminescence response is concerned.

[1]  E. Bröcker,et al.  A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes. , 1985, Journal of immunology.

[2]  A. Feller,et al.  Monocyte/macrophage-specific monoclonal antibody Ki-M1 recognizes interdigitating reticulum cells. , 1984, The American journal of pathology.

[3]  H. Radzun,et al.  Immunoelectron microscopic demonstration of tissue antigens with monoclonal antibodies , 1984, Virchows Archiv. B, Cell pathology including molecular pathology.

[4]  L. Lachman,et al.  Control of human lymphocyte proliferation by soluble factors. , 1984, Laboratory investigation; a journal of technical methods and pathology.

[5]  E. Unanue,et al.  Antigen presentation: comments on its regulation and mechanism. , 1984, Journal of immunology.

[6]  S. Wahl,et al.  Characterization of hydrogen peroxide-potentiating factor, a lymphokine that increases the capacity of human monocytes and monocyte-like cell lines to produce hydrogen peroxide. , 1983, Journal of immunology.

[7]  H. Zola,et al.  The reactivity of a monoclonal antibody against cells of the monocyte-macrophage series in sections of normal and inflamed human tissues. , 1983, Journal of the Reticuloendothelial Society.

[8]  H. Radzun,et al.  Differential immunohistochemical resolution of the human mononuclear phagocyte system. , 1983, Cellular immunology.

[9]  D. Hume,et al.  The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs , 1983, The Journal of experimental medicine.

[10]  R. Steinman,et al.  Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages , 1983, The Journal of experimental medicine.

[11]  R. Steinman,et al.  Relative efficacy of human monocytes and dendritic cells as accessory cells for T cell replication , 1983, The Journal of experimental medicine.

[12]  M. Fanger,et al.  The expression of myeloid-specific antigens on myeloid leukemia cells: correlations with leukemia subclasses and implications for normal myeloid differentiation. , 1983, Blood.

[13]  H. Radzun,et al.  Monocytic origin of the human hematopoietic cell line U‐937 and its convertibility to macrophages evidenced by isoenzyme mapping , 1983, International journal of cancer.

[14]  H. Radzun,et al.  Monocytic origin of human alveolar macrophages. , 1983, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[15]  R. Winchester,et al.  Human mononuclear phagocyte differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. , 1983, Journal of immunology.

[16]  H. Stein,et al.  Human dendritic reticulum cells of lymphoid follicles: Their antigenic profile and their identification as multinucleated giant cells , 1983, Virchows Archiv. B, Cell pathology including molecular pathology.

[17]  H. Radzun,et al.  Monoclonal antibody Ki-M4 specifically recognizes human dendritic reticulum cells (follicular dendritic cells) and their possible precursor in blood. , 1983, Blood.

[18]  R. Atkins,et al.  Antigenic heterogeneity of human mononuclear phagocytes: immunohistologic analysis using monoclonal antibodies. , 1983, Blood.

[19]  V. Diehl,et al.  Identification of Hodgkin and sternberg‐reed cells as a unique cell type derived from a newly‐detected small‐cell population , 1982, International journal of cancer.

[20]  J. Kearney,et al.  A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. , 1982, Clinical immunology and immunopathology.

[21]  R. Steinman,et al.  Human dendritic cells. Enrichment and characterization from peripheral blood , 1982, The Journal of experimental medicine.

[22]  R. Andrews,et al.  Normal and malignant human myelocytic and monocytic cells identified by monoclonal antibodies. , 1982, Journal of immunology.

[23]  R. Steinman,et al.  Dendritic cells in the immune response: characteristics and recommended nomenclature (A report from the Reticuloendothelial Society Committee on Nomenclature). , 1982, Journal of the Reticuloendothelial Society.

[24]  E. Hoefsmit,et al.  Relation between langerhans cells, veiled cells, and interdigitating cells. , 1982, Immunobiology.

[25]  J. Shelhamer,et al.  Characterization of a monoclonal antibody (4F2) that binds to human monocytes and to a subset of activated lymphocytes. , 1981, Journal of immunology.

[26]  L. Nadler,et al.  Antigens on human monocytes identified by monoclonal antibodies. , 1981, Journal of immunology.

[27]  P. Lipsky,et al.  A monoclonal antibody that identifies human peripheral blood monocytes recognizes the accessory- cells required for mitogen-induced T lymphocyte proliferation. , 1981, Journal of immunology.

[28]  H. Radzun,et al.  The homogeneity and monocytic origin of human peritoneal macrophages evidence by comparison of esterase polymorphism. , 1981, The American journal of pathology.

[29]  R. van Furth.  Current view of the mononuclear phagocyte system. , 1981, Haematology and blood transfusion.

[30]  T. Lebien,et al.  A monoclonal antibody (TA-1) reactive with human T lymphocytes and monocytes. , 1980, Journal of immunology.

[31]  J. D. Capra,et al.  Initial characterization of monoclonal antibodies against human monocytes. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[32]  L. Picker,et al.  T cells and macrophages involved in the autologous mixed lymphocyte reaction are required for the response to conventional antigen. , 1980, Journal of immunology.

[33]  L. Picker,et al.  Macrophage heterogeneity in man. A subpopulation of HLA-DR-bearing macrophages required for antigen-induced T cell activation also contains stimulators for autologous-reactive T cells , 1980, The Journal of experimental medicine.

[34]  K. Lennert,et al.  Immunohistologic analysis of the organization of normal lymphoid tissue and non-Hodgkin's lymphomas. , 1980, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[35]  T. Flotte,et al.  Langerhans cells as macrophages in skin and lymphoid organs. , 1980, The Journal of investigative dermatology.

[36]  H. Radzun,et al.  Lysosomal acid esterase: activity and isoenzymes in separated normal human blood cells. , 1980, Blood.

[37]  E. Reinherz,et al.  A monoclonal antibody reactive with human peripheral blood monocytes. , 1980, Journal of immunology.

[38]  E. Reinherz,et al.  Monoclonal antibodies defining distinctive human T cell surface antigens. , 1979, Science.

[39]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[40]  C. Damsky,et al.  Human promyelocytic leukemia cells in culture differentiate into macrophage-like cells when treated with a phorbol diester. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[41]  S. Collins,et al.  Normal functional characteristics of cultured human promyelocytic leukemia cells (HL-60) after induction of differentiation by dimethylsulfoxide , 1979, The Journal of experimental medicine.

[42]  E. Unanue.  Secretory function of mononuclear phagocytes: a review. , 1976, The American journal of pathology.

[43]  C. Milstein,et al.  Continuous cultures of fused cells secreting antibody of predefined specificity , 1975, Nature.

[44]  S. Silverstein,et al.  Studies of the macrophage complement receptor. Alteration of receptor function upon macrophage activation , 1975, The Journal of experimental medicine.

[45]  R. Steinman,et al.  IDENTIFICATION OF A NOVEL CELL TYPE IN PERIPHERAL LYMPHOID ORGANS OF MICE , 1974, The Journal of experimental medicine.

[46]  W. Daems,et al.  PEROXIDATIC ACTIVITY IN RESIDENT PERITONEAL MACROPHAGES AND EXUDATE MONOCYTES OF THE GUINEA PIG AFTER INGESTION OF LATEX PARTICLES , 1973, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[47]  W. Crosby,et al.  Cytochemical identification of monocytes and granulocytes. , 1971, American journal of clinical pathology.

[48]  L. Rather,et al.  Lectures on the Comparative Pathology of Inflammation , 1970, Medical History.

[49]  A. Zelickson,et al.  Epidermal dendritic cells. A quantitative study. , 1968, Archives of dermatology.

[50]  G. Nossal,et al.  Antigens in immunity. XV. Ultrastructural features of antigen capture in primary and secondary lymphoid follicles. , 1968 .

[51]  G. Nossal,et al.  ANTIGENS IN IMMUNITY , 1968, The Journal of experimental medicine.