LIPASE-CATALYZED BIODIESEL SYNTHESIS WITH DIFFERENT ACYL ACCEPTORS

Biodiesel is an alternative fuel for diesel engine that is environmentally acceptable. Conventionally, biodiesel is produced by transesterification of triglycerides and short alcohols in the presence of an acid or an alkaline catalyst. There are several problems associated with this kind of production that can be resolved by using lipase as the biocatalyst. The aim of the present work was to investigate novel acyl acceptors for biodiesel production. 2-Propanol and n-butanol have a less negative effect on lipase stability, and they also improve low temperature properties of the fuel. However, excess alcohol leads to inactivation of the enzyme, and glycerol, a major byproduct, can block the immobilized enzyme, resulting in low enzymatic activity. This problem was solved by using methyl acetate as acyl acceptor. Triacetylglycerol is produced instead of glycerol, and it has no negative effect on the activity of the lipase.

[1]  A. Salis,et al.  Biodiesel production from triolein and short chain alcohols through biocatalysis. , 2005, Journal of biotechnology.

[2]  H. von Blottnitz,et al.  A life-cycle comparison between inorganic and biological catalysis for the production of biodiesel , 2008 .

[3]  Dehua Liu,et al.  Study on the kinetics of enzymatic interesterification of triglycerides for biodiesel production with methyl acetate as the acyl acceptor , 2005 .

[4]  Fang Wang,et al.  Lipase catalyzed methanolysis to produce biodiesel： Optimization of the biodiesel production , 2006 .

[5]  Dehua Liu,et al.  Comparative study on lipase-catalyzed transformation of soybean oil for biodiesel production with different acyl acceptors , 2004 .

[6]  A. Schwab,et al.  Preparation and properties of diesel fuels from vegetable oils , 1987 .

[7]  Sulaiman Al‐Zuhair,et al.  Production of biodiesel: possibilities and challenges , 2007 .

[8]  Yomi Watanabe,et al.  Enzymatic alcoholysis for biodiesel fuel production and application of the reaction to oil processing , 2002 .

[9]  Yomi Watanabe,et al.  Production of FAME from acid oil model using immobilized Candida antarctica lipase , 2005 .

[10]  M. Tüter,et al.  Immobilized Candida antarctica lipase-catalyzed alcoholysis of cotton seed oil in a solvent-free medium. , 2002, Bioresource technology.

[11]  Uwe T. Bornscheuer,et al.  Improvement in lipase-catalyzed synthesis of fatty acid methyl esters from sunflower oil , 2003 .

[12]  Yomi Watanabe,et al.  Conversion of acid oil by-produced in vegetable oil refining to biodiesel fuel by immobilized Candida antarctica lipase , 2007 .

[13]  R. Prasad,et al.  Lipase-mediated conversion of vegetable oils into biodiesel using ethyl acetate as acyl acceptor. , 2007, Bioresource technology.

[14]  D. Royon,et al.  Enzymatic production of biodiesel from cotton seed oil using t-butanol as a solvent. , 2007, Bioresource technology.

[15]  Li Wang,et al.  Lipase-catalyzed transesterification of rapeseed oils for biodiesel production with a novel organic solvent as the reaction medium , 2006 .

[16]  Shweta Shah,et al.  Lipase catalyzed preparation of biodiesel from Jatropha oil in a solvent free system. , 2007 .