Production of L-amino acids by aminoacylase adsorbed on DEAE-Sephadex.

Publisher Summary This chapter discusses the preparation, the enzymic properties, and the industrial application of immobilized aminoacylases. Various immobilization methods were investigated, and relatively active and stable immobilized aminoacylases were obtained by ionic binding to DEAE-Sephadex, covalent binding to iodoacetylcellulose, and entrapping into polyacrylamide gel lattices. Typical immobilization methods for mold aminoacylase are described in the chapter. To store the immobilized preparation for a long period or to measure its enzyme activity, it is suspended in 2.5 liters of distilled water and lyophilized. Using this procedure, 106 g of DEAE-Sephadexaminoacylase is obtained. The activity of the preparation is about 700 moles/hr per gram of preparation under standard assay conditions. The optimum pH of the DEAE-Sephadex-aminoacylase shifts about 0.5–1.0 pH unit more to the acid side than that of the native enzyme. This shift may be explained by the redistribution of hydrogen ions between the positively charged DEAE-Sephadex and the surrounding aqueous medium. This shift is also found for polyacrylamide-aminoacylase, but the reason is not clear in this ease.

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