Analysis of Ginsenosides from Panax quinquefolium L. by Automated Multiple Development

Conditions have been established for separation of eight ginsenosides by HPTLC with automated multiple development. The ginsenosides were separated on silica gel 60F254 with methanol-chloro-form mobile phases. Two gradiënt programs and two different values of the increment in the development distance were compared. Factors affecting extraction of the ginsenosides from leaves were investigated. The results show that AMD is suitable for screening of ginsenosides in extracts from ginseng.