Simultaneous saccharification and fermentation of potato starch wastewater to lactic acid by Rhizopus oryzae and Rhizopus arrhizus

The biochemical kinetic of simultaneous saccharification and fermentation (SSF) for lactic acid production by fungal species of Rhizopus arrhizus 36017 and Rhizopus oryzae 2062 was studied with respect to growth pH, temperature and substrate. Both R. arrhizus 36017 and R. oryzae 2062 had a capacity to carry out a single stage SSF process for lactic acid production from potato starch wastewater. The kinetic characteristics, termed as starch hydrolysis, accumulation of reducing sugars, lactic acid production and fungal biomass formation, were affected with variations in pH, temperature, and starch source and concentration. A growth condition with starch concentration approximately 20 g/l at pH 6.0 and 30 degrees C was favourable for both starch saccharification and lactic acid fermentation, resulting in lactic acid yield of 0.85-0.92 g/g associated with 1.5-3.5 g/l fungal biomass produced in 36-48 h fermentation. R. arrhizus 36017 had a higher capacity to produce lactic acid, while R. oryzae 2062 produced more fungal biomass under similar conditions. (c) 2005 Elsevier B.V. All rights reserved.

[2]  R. E. Mueller,et al.  Lactic acid production from enzyme-thinned corn starch usingLactobacillus amylovorus , 2005, Journal of Industrial Microbiology.

[3]  M. Akkaya,et al.  RT-PCR amplification of a Rhizopus oryzae lactate dehydrogenase gene fragment. , 2001, Enzyme and microbial technology.

[4]  Y. Oda,et al.  Lactic Acid Fermentation of Potato Pulp by the Fungus Rhizopus oryzae , 2002, Current Microbiology.

[5]  M. Penttilä,et al.  Metabolic engineering applications to renewable resource utilization. , 2000, Current opinion in biotechnology.

[6]  G. Zacchi,et al.  Simultaneous enzymatic wheat starch saccharification and fermentation to lactic acid by Lactococcus lactis , 1999, Applied Microbiology and Biotechnology.

[7]  Ana Belén Moldes,et al.  Strategies to improve the bioconversion of processed wood into lactic acid by simultaneous saccharification and fermentation , 2001 .

[8]  C. Berthold,et al.  Biotechnological Conversion of Sugar and Starchy Crops into Lactic Acid , 1998 .

[9]  S. Tsai,et al.  An integrated bioconversion process for production of L-lactic acid from starchy potato feedstocks. , 1998 .

[10]  M. Galbe,et al.  The influence of lactic acid formation on the simultaneous saccharification and fermentation (SSF) of softwood to ethanol , 2000 .

[11]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[12]  L. Lynd,et al.  Fuel Ethanol from Cellulosic Biomass , 1991, Science.

[13]  Bo Jin,et al.  A Bioprocessing Mode for Simultaneous Fungal Biomass Protein Production and Wastewater Treatment Using an External Air-Lift Bioreactor , 2001 .

[14]  G. T. Tsao,et al.  Optimization of L-Lactic acid production from glucose by Rhizopus oryzae ATCC 52311 , 1999, Applied biochemistry and biotechnology.

[15]  Munir Cheryan,et al.  Starch to lactic acid in a continuous membrane bioreactor , 1994 .

[16]  E. Woodams,et al.  Production of L(+)-lactic acid byRhizopus oryzae immobilized in calcium alginate gels , 1989, Biotechnology Letters.

[17]  M. Okabe,et al.  Enhanced production of L(+)-lactic acid from corn starch in a culture of Rhizopus oryzae using an air-lift bioreactor , 1997 .

[18]  C. R. Soccol,et al.  Production of l-lactic acid by Rhizopus species , 1994, World journal of microbiology & biotechnology.

[19]  G. Zacchi,et al.  Modelling the influence of pH, temperature, glucose and lactic acid concentrations on the kinetics of lactic acid production by Lactococcus lactis ssp. lactis ATCC 19435 in whole-wheat flour , 1998, Applied Microbiology and Biotechnology.

[20]  C. Gong,et al.  Production of multifunctional organic acids from renewable resources. , 1999, Advances in biochemical engineering/biotechnology.

[21]  P. Lant,et al.  Biotechnological production of lactic acid integrated with potato wastewater treatment by Rhizopus arrhizus , 2003 .

[22]  Akkihebbal K. Suresh,et al.  Simultaneous saccharification and fermentation of starch to lactic acid , 1999 .

[23]  Y. Y. Lee,et al.  Product inhibition in simultaneous saccharification and fermentation of cellulose into lactic acid , 1999, Biotechnology Letters.

[24]  Y. Linko,et al.  Simultaneous liquefaction, saccharification, and lactic acid fermentation on barley starch , 1996 .

[25]  P. Lant,et al.  Rhizopus arrhizus – a producer for simultaneous saccharification and fermentation of starch waste materials to l(+)-lactic acid , 2003, Biotechnology Letters.

[26]  L. C. Thomas,et al.  Colorimetric chemical analytical methods , 1974 .

[27]  Hofvendahl,et al.  Factors affecting the fermentative lactic acid production from renewable resources(1). , 2000, Enzyme and microbial technology.

[28]  G. Reddy,et al.  Direct fermentation of various pure and crude starchy substrates to L(+) lactic acid using Lactobacillus amylophilus GV6 , 2002 .

[29]  J. R. Frank,et al.  Technological and economic potential of poly(lactic acid) and lactic acid derivatives , 1995 .

[30]  B. Patel,et al.  Production of fungal protein and glucoamylase by Rhizopus oligosporus from starch processing wastewater , 1999 .

[31]  Christos Hatzis,et al.  Biochemical Engineering Analysis of Critical Process Factors in the Biomass‐to‐Ethanol Technology , 1997, Biotechnology progress.

[32]  M. Rosenberg,et al.  Physiological restriction of the L‐lactic acid production by Rhizopus arrhizus , 1995 .

[33]  Y. D. Hang,et al.  Kinetics of direct fermentation of agricultural commodities to L(+) lactic acid byRhizopus oryzae , 1989, Biotechnology Letters.