Five-dimensional optoacoustic tomography for real-time whole brain neuroimaging of stimulus-evoked responses

We present a technique for fully noninvasive acquisition of real-time volumetric multispectral optoacoustic data from whole mouse brain. The neuroimaging capacity of the new methodology is demonstrated here by simultaneous label-free assessment of multiple stimulus-evoked hemodynamic responses, including blood oxygenation, total hemoglobin, cerebral blood volume, oxygenized and deoxygenized hemoglobin.