Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy

We discuss unique features of lens-free computational imaging tools and report some of their emerging results for wide-field on-chip microscopy, such as the achievement of a numerical aperture (NA) of ∼0.8–0.9 across a field of view (FOV) of more than 20 mm2 or an NA of ∼0.1 across a FOV of ∼18 cm2, which corresponds to an image with more than 1.5 gigapixels. We also discuss the current challenges that these computational on-chip microscopes face, shedding light on their future directions and applications.

[1]  Angela Saini Microscopy. New lens offers scientist a brighter outlook. , 2012, Science.

[2]  W. Bishara,et al.  Lens-free optical tomographic microscope with a large imaging volume on a chip , 2011, Proceedings of the National Academy of Sciences.

[3]  H. Kreuzer,et al.  Immersion digital in-line holographic microscopy. , 2006, Optics letters.

[4]  Hongying Zhu,et al.  Optofluidic Tomography on a Chip. , 2011, Applied physics letters.

[5]  P. Marquet,et al.  Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba. , 2006, Optics express.

[6]  Derek Tseng,et al.  Lensfree on-chip imaging using nanostructured surfaces. , 2010, Applied physics letters.

[7]  Guoan Zheng,et al.  Color sub-pixel resolving optofluidic microscope and its application to blood cell imaging for malaria diagnosis , 2011 .

[8]  J. Miao,et al.  Phase retrieval from the magnitude of the Fourier transforms of nonperiodic objects , 1998 .

[9]  Michael Elad,et al.  A fast super-resolution reconstruction algorithm for pure translational motion and common space-invariant blur , 2001, IEEE Trans. Image Process..

[10]  G. Pedrini,et al.  Phase retrieval of arbitrary complex-valued fields through aperture-plane modulation , 2007 .

[11]  M. Snir,et al.  Big data, but are we ready? , 2011, Nature Reviews Genetics.

[12]  Peter Klages,et al.  Digital in-line holographic microscopy. , 2006, Applied optics.

[13]  Leslie J. Allen,et al.  Phase retrieval from series of images obtained by defocus variation , 2001 .

[14]  J. Rodenburg,et al.  Ptychographic electron microscopy using high-angle dark-field scattering for sub-nanometre resolution imaging , 2012, Nature Communications.

[15]  Derek K. Tseng,et al.  Compact and light-weight automated semen analysis platform using lensfree on-chip microscopy. , 2010, Analytical chemistry.

[16]  K. Nugent Coherent methods in the X-ray sciences , 2009, 0908.3064.

[17]  Aydogan Ozcan,et al.  Lensfree Fluorescent On-Chip Imaging of Transgenic Caenorhabditis elegans Over an Ultra-Wide Field-of-View , 2011, PloS one.

[18]  O. Bunk,et al.  Ptychographic X-ray computed tomography at the nanoscale , 2010, Nature.

[19]  Derek Tseng,et al.  Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications. , 2010, Lab on a chip.

[20]  J. Miao,et al.  Extending the methodology of X-ray crystallography to allow imaging of micrometre-sized non-crystalline specimens , 1999, Nature.

[21]  Werner Jüptner,et al.  Digital recording and numerical reconstruction of holograms , 2002 .

[22]  F. Dubois,et al.  Improved three-dimensional imaging with a digital holography microscope with a source of partial spatial coherence. , 1999, Applied optics.

[23]  J. Miao,et al.  The oversampling phasing method. , 2000, Acta crystallographica. Section D, Biological crystallography.

[24]  Aydogan Ozcan,et al.  Lensfree super-resolution holographic microscopy using wetting films on a chip , 2011, Optics express.

[25]  R Riesenberg,et al.  Reconstruction of high-resolution holographic microscopic images. , 2009, Optics letters.

[26]  Christophe Ducottet,et al.  Numerical suppression of the twin image in in-line holography of a volume of micro-objects , 2008 .

[27]  Toyohiko Yatagai,et al.  Doppler phase-shifting digital holography and its application to surface shape measurement. , 2010, Optics letters.

[28]  Edward A. Watson,et al.  High-Resolution Image Reconstruction from a Sequence of Rotated and Translated Frames and its Application to an Infrared Imaging System , 1998 .

[29]  Tomoyoshi Shimobaba,et al.  Computational wave optics library for C++: CWO++ library , 2011, Comput. Phys. Commun..

[30]  Fucai Zhang,et al.  Superresolution imaging via ptychography. , 2011, Journal of the Optical Society of America. A, Optics, image science, and vision.

[31]  Daniel L Marks,et al.  Compressive holography. , 2009, Optics express.

[32]  C. Simmons,et al.  A digital microfluidic platform for primary cell culture and analysis. , 2012, Lab on a chip.

[33]  J R Fienup,et al.  Phase retrieval algorithms: a comparison. , 1982, Applied optics.

[34]  Keith A. Nugent,et al.  Coherent lensless X-ray imaging , 2010 .

[35]  Demetri Psaltis,et al.  Lensless high-resolution on-chip optofluidic microscopes for Caenorhabditis elegans and cell imaging , 2008, Proceedings of the National Academy of Sciences.

[36]  Steve Feng,et al.  Distributed Medical Image Analysis and Diagnosis through Crowd-Sourced Games: A Malaria Case Study , 2012, PloS one.

[37]  B. Javidi,et al.  Compressive Fresnel Holography , 2010, Journal of Display Technology.

[38]  B. Javidi,et al.  Synthetic aperture single-exposure on-axis digital holography. , 2008, Optics express.

[39]  Moon Gi Kang,et al.  Super-resolution image reconstruction: a technical overview , 2003, IEEE Signal Process. Mag..

[40]  Guoan Zheng,et al.  The ePetri dish, an on-chip cell imaging platform based on subpixel perspective sweeping microscopy (SPSM) , 2011, Proceedings of the National Academy of Sciences.

[41]  Aydogan Ozcan,et al.  Lensless wide-field fluorescent imaging on a chip using compressive decoding of sparse objects , 2010, Optics express.

[42]  Aydogan Ozcan,et al.  Field-portable reflection and transmission microscopy based on lensless holography , 2011, Biomedical optics express.

[43]  J. Miao,et al.  Application of optimization technique to noncrystalline x-ray diffraction microscopy: Guided hybrid input-output method , 2007 .

[44]  Changhuei Yang,et al.  Implementation of a color-capable optofluidic microscope on a RGB CMOS color sensor chip substrate. , 2010, Lab on a chip.

[45]  Thomas Otto,et al.  Highly-integrated lab-on-chip system for point-of-care multiparameter analysis. , 2012, Lab on a chip.

[46]  L. Repetto,et al.  Lensless digital holographic microscope with light-emitting diode illumination. , 2004, Optics letters.

[47]  A. Ozcan,et al.  Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array. , 2011, Lab on a chip.

[48]  Rick P. Millane,et al.  Phase retrieval in crystallography and optics , 1990 .

[49]  P. Thibault Algorithmic methods in diffraction microscopy , 2007 .

[50]  Changhuei Yang,et al.  Fluorescence microscopy imaging with a Fresnel zone plate array based optofluidic microscope. , 2011, Lab on a chip.

[51]  A. Ozcan,et al.  Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution , 2010, Optics express.

[52]  Garth J. Williams,et al.  Lensless imaging using broadband X-ray sources , 2011 .

[53]  Aydogan Ozcan,et al.  Field-portable wide-field microscopy of dense samples using multi-height pixel super-resolution based lensfree imaging. , 2012, Lab on a chip.

[54]  Paul Petruck,et al.  High resolution (NA = 0.8) in lensless in-line holographic microscopy with glass sample carriers. , 2011, Optics letters.

[55]  D. Brady,et al.  Video-rate compressive holographic microscopic tomography. , 2011, Optics express.

[56]  A. Ozcan,et al.  Lensfree holographic imaging of antibody microarrays for high-throughput detection of leukocyte numbers and function. , 2010, Analytical chemistry.

[57]  Aydogan Ozcan,et al.  Wide-field lensless fluorescent microscopy using a tapered fiber-optic faceplate on a chip. , 2011, The Analyst.

[58]  E. Cuche,et al.  Spatial filtering for zero-order and twin-image elimination in digital off-axis holography. , 2000, Applied optics.

[59]  J. Rodenburg,et al.  Optical ptychography: a practical implementation with useful resolution. , 2010, Optics letters.

[60]  Hongying Zhu,et al.  Holographic opto-fluidic microscopy , 2010, Optics express.