Innovative biorefinery concept for sugar-based ethanol industries: production of protein-rich fungal biomass on vinasse as an aquaculture feed ingredient.

This study examined the potential of producing a protein-rich fungus, Rhizopus microsporus (var. oligosporus), as an aquaculture feed ingredient on vinasse generated during sugar-based ethanol fermentation. Optimization studies showed prolific fungal growth at pH 5.0 and 30 degrees C on vinasse with nutrient (nitrogen and phosphorus) supplementation. The molasses-vinasse resulted in the highest specific fungal biomass yield of 0.21 (g biomass increase/(g initial biomassxg soluble chemical oxygen demand (SCOD) removed)). Organic and inorganic matters were significantly reduced, e.g., 42.02% SCOD and 24.41% total dissolved solids. Thus, the treated effluent could be recycled as process water or used for land applications. The fungal biomass was found to have 45.55% crude protein with significantly high amino acids, arginine and threonine. The integration of innovative fungal technology with sugar-based ethanol production could provide an opportunity for producing food-grade fungal protein for animal feed application with simultaneous water reclamation.

[1]  G. Khor,et al.  Conversion of carbohydrates to protein by high temperature fungi , 1976 .

[2]  Samir Kumar Khanal,et al.  Anaerobic Biotechnology for Bioenergy Production , 2008 .

[3]  Fu-I Lu,et al.  Effect of exogenous tryptophan on cannibalism, survival and growth in juvenile grouper, Epinephelus coioides , 2003 .

[4]  K. El-Shazly,et al.  Effect of essential amino acids (methionine and lysine) and treated oil in fish diet on growth performance and feed utilization of Nile tilapia, Tilapia nilotica (L.) , 1993 .

[5]  Samir Kumar Khanal,et al.  Anaerobic Biotechnology for Bioenergy Production: Principles and Applications , 2008 .

[6]  Ø. Øverli,et al.  Suppression of aggression in rainbow trout (Oncorhynchus mykiss) by dietary L-tryptophan. , 2001, The Journal of experimental biology.

[7]  R. Ruiz,et al.  Determination of Sugars, Byproducts, and Degradation Products in Liquid Fraction Process Samples , 2008 .

[8]  Kenneth Helrich,et al.  Official methods of analysis of the Association of Official Analytical Chemists , 1990 .

[9]  L. Lange,et al.  Advances in Fungal Biotechnology for Industry, Agriculture, and Medicine , 2012, Springer US.

[10]  Gartenbau Forstwirtschaft Fischerei Ernährung Landwirtschaft.  Institute of Food and Agricultural Sciences , 2011 .

[11]  Board on Agriculture,et al.  Nutrient requirements of swine , 1964 .

[12]  A. E. Greenberg,et al.  Standard Methods for the Examination of Water and Wastewater seventh edition , 2013 .

[13]  Beatrice Gralton,et al.  Washington DC - USA , 2008 .

[14]  Michael H. Schwarz,et al.  Understanding Fish Nutrition, Feeds, and Feeding , 2009 .

[15]  J. Goldemberg,et al.  The Sustainability of Ethanol Production from Sugarcane , 2008, Renewable Energy.

[16]  R. D. Miles,et al.  The Benefits of Fish Meal in Aquaculture Diets 1 , 2012 .

[17]  Kelly,et al.  Biocatalysis and biotransformation , 1999, Current opinion in chemical biology.

[18]  S. Khanal,et al.  Converting corn wet‐milling effluent into high‐value fungal biomass in a biofilm reactor , 2008, Biotechnology and Bioengineering.