Morphological anomalies in somatic embryo structure in Catharanthus roseus : Improving embryo germination by amending plant growth regulators , activated charcoal and sucrose level

The present study describes the incidence of somatic embryo (SE) irregularities in Catharanthus roseus. In this ornamental and anti-cancerous medicinal plant, SEs were developed at a very high rate from hypocotyl callus on MS medium, amended with 1.0 mg l naphthalene acetic acid (NAA) and 1.5 mg l benzylaminopurine (BA). But at high (1.5 mg l) plant growth regulators (PGRs) levels severe embryo developmental abnormalities were caused. The major aberrant SE types have been described in the present communication. In several cases, the in vitro raised SEs had underdeveloped root ends or with aborted root axis. These irregularities in embryo structure appreciably reduced embryo germination rate and plantlet recovery. Here, we evaluated the effect of various PGRs, activated charcoal (AC), and various levels of sucrose in order to improve SE quality and germination. Medium added with 4.0 % sucrose improved rooting ability and growth. The addition of AC improved plantlet conversion significantly by promoting rooting.

[1]  K. Manoharan,et al.  Effect of polyethylene glycol and mannitol on somatic embryogenesis of pigeonpea, Cajanus cajan (L.) Millsp. , 2012 .

[2]  L. Charles,et al.  Enhancement of maturation and germination of somatic embryos in Persian walnut (Juglans regia l.) using osmolites, hormones and cold treatments , 2010 .

[3]  J. Burns,et al.  Development and germination of pecan somatic embryos derived from liquid culture , 1995, In Vitro Cellular & Developmental Biology - Plant.

[4]  K. Ishii,et al.  Somatic embryogenesis and plant regeneration in yakutanegoyou, Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, an endemic and endangered species in Japan , 2007, In Vitro Cellular & Developmental Biology - Plant.

[5]  A. Mujib,et al.  Somatic embryo proliferation, maturation and germination in Catharanthus roseus , 2006, Plant Cell, Tissue and Organ Culture.

[6]  H. Ezura,et al.  Efficient plant regeneration and Agrobacterium-mediated transformation via somatic embryogenesis in melon (Cucumis melo L.) , 2004 .

[7]  G. Bellocchi,et al.  Effect of light quality on somatic embryogenesis of quince leaves , 1998, Plant Cell, Tissue and Organ Culture.

[8]  D. Blakesley,et al.  Uptake and metabolism of 6-benzyladenine in shoot cultures of a range of species , 1992, Plant Cell, Tissue and Organ Culture.

[9]  Elisa Quiala,et al.  Effect of dissolved oxygen concentration on differentiation of somatic embryos of Coffea arabica cv. Catimor 9722 , 2004, Plant Cell, Tissue and Organ Culture.

[10]  H. V. Van Onckelen,et al.  The Role of Auxin, pH, and Stress in the Activation of Embryogenic Cell Division in Leaf Protoplast-Derived Cells of Alfalfa1 , 2002, Plant Physiology.

[11]  Wei Tang,et al.  Plant regeneration from embryogenic cultures initiated from mature loblolly pine zygotic embryos , 2001, In Vitro Cellular & Developmental Biology - Plant.

[12]  C. Bastien,et al.  Somatic embryogenesis and plantlet development in Pinus sylvestris and Pinus pinaster on medium with and without growth regulators , 1999 .

[13]  R. Meagher,et al.  Expression of foreign genes in transgenic yellow-poplar plants. , 1992, Plant physiology.

[14]  T. Eriksson,et al.  Effects of Activated Charcoal on Growth and Morphogenesis in Cell Cultures , 1975 .