Ethanol Production from the Stem Juice of Different Sweet Sorghum Cultivars in the State of Pernambuco, Northeast of Brazil

The decline in the use of fossil fuels in the future is due to the gradual depletion of their reserves and related environmental issues. Biofuels including ethanol are presented as a renewable alternative to fossil fuels. However, ethanol production, generally from sugarcane demands the use of agricultural land and other natural resources, with emphasis on water and other nutrients. Sorghum is tolerant to drought. The purpose of this study was to evaluate the potentiality of eight sweet sorghum cultivars in order to expand the ethanol production to areas with limited supply of water, not suitable for sugarcane which requires irrigation. The cultivars developed by breeding programs in the state of Pernambuco, Brazil, were analyzed for fermentation by industrial strain of Saccharomyces cerevisiae, at 33 °C, under static conditions for a period of 6 h. All assessed parameters showed some statistically significant differences among cultivars (p < 0.01), especially ethanol concentration (P) and the volumetric productivities (Qp) in the following order: Willey (P = 64.77 ± 4.4 g L−1 and Qp = 10.79 ± 0.74 g L−1 h−1), Wray (P = 59.10 ± 1.7 g L−1 and Qp = 9.85 ± 0.28 g L−1 h−1), BR 506 (P = 59.07 ± 1.3 g L−1 and Qp = 9.84 ± 0.21 g L−1 h−1) and SF 15 (54.40 ± 0.4 g L−1 and Qp = 9.07 ± 0.06 g L−1 h−1). The highest potentials for ethanol yield per hectare were observed for the cultivars SF 15 (3142.51 ± 428.47 L ha−1) and BR 506 (2193.95 ± 383.58 L ha−1). Therefore, the cultivars SF 15 and BR 506 seem to be very promising as an energy crop to be produced in areas with environmental conditions similar to Pernambuco.

[1]  P. Greenfield,et al.  A review of yeast ionic nutrition. I: Growth and fermentation requirements , 1984 .

[2]  I. Vasilakoglou,et al.  Sweet sorghum productivity for biofuels under increased soil salinity and reduced irrigation , 2011 .

[3]  L. Laopaiboon,et al.  Growth and batch ethanol fermentation of Saccharomyces cerevisiae on sweet sorghum stem juice under normal and very high gravity conditions. , 2010 .

[4]  A. Almodares,et al.  Production of bioethanol from sweet sorghum: A review , 2009 .

[5]  U. A. Lima,et al.  Biotecnologia industrial ;: processos fermentativos e enzimáticos , 2001 .

[6]  Seth C. Murray,et al.  Genetic Improvement of Sorghum as a Biofuel Feedstock: II. QTL for Stem and Leaf Structural Carbohydrates , 2008 .

[7]  P. Sanjana Reddy,et al.  Sweet Sorghum - A Potential Alternate Raw Material for Bio-ethanol and Bio- energy , 2005 .

[8]  Y. Goto,et al.  Cultivation of Sweet Sorghum (Sorghum bicolor (L.) Moench) and Determination of its Harvest Time to Make Use as the Raw Material for Fermentation, Practiced during Rainy Season in Dry Land of Indonesia , 2004 .

[9]  Francisco de Assis Santos,et al.  VERSÃO DO PROGRAMA COMPUTACIONAL ASSISTAT PARA O SISTEMA OPERACIONAL WINDOWS , 2002 .

[10]  R. W. Sheard,et al.  Comparison of Conventional and Automated Procedures for Nitrogen, Phosphorus, and Potassium Analysis of Plant Material Using a Single Digestion1 , 1967 .

[11]  C. Lareo,et al.  Bioethanol production from sweet sorghum: Evaluation of post-harvest treatments on sugar extraction and fermentation , 2011 .

[12]  M. A. Morais,et al.  Yeast population dynamics of industrial fuel-ethanol fermentation process assessed by PCR-fingerprinting , 2005, Antonie van Leeuwenhoek.

[13]  P. Thanonkeo,et al.  Ethanol production from sweet sorghum juice in batch and fed-batch fermentations by Saccharomyces cerevisiae , 2007 .

[14]  M. Moo-young,et al.  Ethanol fermentation technologies from sugar and starch feedstocks. , 2008, Biotechnology advances.

[15]  William L. Rooney,et al.  Features of sweet sorghum juice and their performance in ethanol fermentation , 2010 .

[16]  W. Rooney,et al.  Evaluation of bioethanol production from five different varieties of sweet and forage sorghums (Sorghum bicolor (L) Moench) , 2011 .

[17]  Shiv Prasad,et al.  Ethanol Production from Sweet Sorghum Syrup for Utilization as Automotive Fuel in India , 2007 .

[18]  N. Seetharama,et al.  Study on genotypic variation for ethanol production from sweet sorghum juice , 2010 .

[19]  G. Walker Metals in yeast fermentation processes. , 2004, Advances in applied microbiology.

[20]  Mark R. Wilkins,et al.  Influence of temperature, pH and yeast on in-field production of ethanol from unsterilized sweet sorghum juice. , 2010 .

[21]  Guang Hui Xie,et al.  Biomass yield and changes in chemical composition of sweet sorghum cultivars grown for biofuel. , 2009 .

[22]  C. Cardona,et al.  Trends in biotechnological production of fuel ethanol from different feedstocks. , 2008, Bioresource technology.

[23]  T. P. Lyons,et al.  The alcohol textbook , 2003 .

[24]  G. L. Miller Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[25]  W. M. Ingledew,et al.  Practical and theoretical considerations in the production of high concentrations of alcohol by fermentation , 1996 .

[26]  A. Busturia,et al.  Mechanisms of appearance of the Pasteur effect in Saccharomyces cerevisiae: inactivation of sugar transport systems , 1982, Journal of bacteriology.

[27]  Henrique Vianna de Amorim,et al.  Sugar cane juice and molasses, beet molasses and sweet sorghum: composition and usage , 2009 .

[28]  G. Walker 125th Anniversary Review: Fuel Alcohol: Current Production and Future Challenges , 2011 .