Biosensing with integrated CMOS nanopores

This paper outlines our recent efforts in using solid-state nanopores as a biosensing platform. Traditionally biosensors concentrate mainly on the detection platform and not on signal processing. This decoupling can lead to inferior sensors and is exacerbated in nanoscale devices, where device noise is large and large dynamic range is required. This paper outlines a novel platform that integrates the nano, micro and macroscales in a closely coupled manner that mitigates many of these problems. We discuss our initial results of DNA translocation through the nanopore. We also briefly discuss the use of molecular recognition properties of aptamers with the versatility of the nanopore detector to design a new class of biosensors in a CMOS compatible platform.

[1]  L. Theogarajan,et al.  Wafer Scale Integration of CMOS Chips for Biomedical Applications via Self-Aligned Masking , 2011, IEEE Transactions on Components, Packaging and Manufacturing Technology.

[2]  Oguz H. Elibol,et al.  Fabrication of a CMOS compatible nanopore detector for DNA , 2010 .

[3]  Li-Qun Gu,et al.  Capturing single molecules of immunoglobulin and ricin with an aptamer-encoded glass nanopore. , 2009, Analytical chemistry.

[4]  Kevin W Plaxco,et al.  High specificity, electrochemical sandwich assays based on single aptamer sequences and suitable for the direct detection of small-molecule targets in blood and other complex matrices. , 2009, Journal of the American Chemical Society.

[5]  H. Bayley,et al.  Continuous base identification for single-molecule nanopore DNA sequencing. , 2009, Nature nanotechnology.

[6]  D. Branton,et al.  The potential and challenges of nanopore sequencing , 2008, Nature Biotechnology.

[7]  R. Stoltenburg,et al.  SELEX--a (r)evolutionary method to generate high-affinity nucleic acid ligands. , 2007, Biomolecular engineering.

[8]  Giorgio Ferrari,et al.  Wide bandwidth transimpedance amplifier for extremely high sensitivity continuous measurements. , 2007, The Review of scientific instruments.

[9]  M. Mascini,et al.  Aptamers-based assays for diagnostics, environmental and food analysis. , 2007, Biomolecular engineering.

[10]  C. Dekker.  Solid-state nanopores. , 2007, Nature nanotechnology.

[11]  H. Bayley,et al.  Toward single molecule DNA sequencing: direct identification of ribonucleoside and deoxyribonucleoside 5'-monophosphates by using an engineered protein nanopore equipped with a molecular adapter. , 2006, Journal of the American Chemical Society.

[12]  D. Branton,et al.  Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules. , 1999, Biophysical journal.

[13]  D. Branton,et al.  Characterization of individual polynucleotide molecules using a membrane channel. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[14]  Carver A. Mead,et al.  Analog VLSI Phototransduction by continuous-time, adaptive, logarithmic photoreceptor circuits , 1995 .

[15]  J. Rabin.  The Retina: An Approachable Part of the Brain , 2013 .

[16]  Zahir M. Hussain,et al.  A Multiplierless DC-Blocker for Single-Bit Sigma-Delta Modulated Signals , 2007, EURASIP J. Adv. Signal Process..