A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase.

In budding yeast genes that encode G1 cyclins and proteins involved in DNA synthesis are transcriptionally activated in late G1. A transcription factor, called SBF, is composed of Swi4 and Swi6 proteins and activates transcription of G1 cyclin genes. A different, but related, complex called MBF binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. MBF contains Swi6 and a 120-kilodalton protein (p120). MBF was purified and the gene encoding p120 (termed MBP1) was cloned. A deletion of MBP1 was not lethal but led to deregulated expression of DNA synthesis genes, indicating a direct regulatory role for MBF in MCB-driven transcription. Mbp1 is related to Swi4. Strains deleted for both MBP1 and SWI4 were inviable, demonstrating that transcriptional activation by MBF and SBF has an important role in the transition from G1 to S phase.

[1]  K Nasmyth,et al.  CLB5 and CLB6, a new pair of B cyclins involved in DNA replication in Saccharomyces cerevisiae. , 1993, Genes & development.

[2]  K Nasmyth,et al.  Transcription factors important for starting the cell cycle in yeast. , 1993, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[3]  K Nasmyth,et al.  Control of the yeast cell cycle by the Cdc28 protein kinase. , 1993, Current opinion in cell biology.

[4]  D. Beach,et al.  Sct1 functions in partnership with Cdc10 in a transcription complex that activates cell cycle START and inhibits differentiation , 1993, Cell.

[5]  L. Breeden,et al.  Analysis of the SWI4/SWI6 protein complex, which directs G1/S-specific transcription in Saccharomyces cerevisiae , 1993, Molecular and cellular biology.

[6]  L. Moore,et al.  Interaction of the yeast Swi4 and Swi6 cell cycle regulatory proteins in vitro. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[7]  K. Tanaka,et al.  A new cdc gene required for S phase entry of Schizosaccharomyces pombe encodes a protein similar to the cdc 10+ and SWI4 gene products. , 1992, The EMBO journal.

[8]  J. Nevins,et al.  E2F: a link between the Rb tumor suppressor protein and viral oncoproteins. , 1992, Science.

[9]  Jianguo Wu,et al.  StuA is required for cell pattern formation in Aspergillus. , 1992, Genes & development.

[10]  F. Cross,et al.  CLB5: a novel B cyclin from budding yeast with a role in S phase. , 1992, Genes & development.

[11]  Michael Primig,et al.  Anatomy of a transcription factor important for the Start of the cell cycle in Saccharomyces cerevisiae , 1992, Nature.

[12]  Kim Nasmyth,et al.  A central role for SWI6 in modulating cell cycle Start-specific transcription in yeast , 1992, Nature.

[13]  L. Breeden,et al.  SWI6 protein is required for transcription of the periodically expressed DNA synthesis genes in budding yeast , 1992, Nature.

[14]  P. Russell,et al.  Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division. , 1992, The EMBO journal.

[15]  L. Johnston,et al.  Cell cycle control of DNA synthesis in budding yeast. , 1992, Nucleic acids research.

[16]  V. Bennett,et al.  The ANK repeat: a ubiquitous motif involved in macromolecular recognition. , 1992, Trends in cell biology.

[17]  L. Johnston,et al.  Control of DNA synthesis genes in fission yeast by the cell-cycle gene cdclO + , 1992, Nature.

[18]  S. Reed,et al.  Isolation of three novel human cyclins by rescue of G1 cyclin (cln) function in yeast , 1991, Cell.

[19]  Brenda J. Andrews,et al.  Transcriptional activation of CLN1, CLN2, and a putative new G1 cyclin (HCS26) by SWI4, a positive regulator of G1-specific transcription , 1991, Cell.

[20]  Kim Nasmyth,et al.  The role of SWI4 and SWI6 in the activity of G1 cyclins in yeast , 1991, Cell.

[21]  S. Rabindran,et al.  Molecular cloning and expression of a human heat shock factor, HSF1. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[22]  Kim Nasmyth,et al.  Positive feedback in the activation of Gl cyclins in yeast , 1991, Nature.

[23]  F. Cross,et al.  A potential positive feedback loop controlling CLN1 and CLN2 gene expression at the start of the yeast cell cycle , 1991, Cell.

[24]  K Nasmyth,et al.  A general approach to the isolation of cell cycle-regulated genes in the budding yeast, Saccharomyces cerevisiae. , 1991, Journal of molecular biology.

[25]  L. Johnston,et al.  Coordination of expression of DNA synthesis genes in budding yeast by a cell-cycle regulated trans factor , 1991, Nature.

[26]  H. Pelham,et al.  A conserved heptapeptide restrains the activity of the yeast heat shock transcription factor. , 1991, The EMBO journal.

[27]  R. Storms,et al.  Characterization of a short, cis-acting DNA sequence which conveys cell cycle stage-dependent transcription in Saccharomyces cerevisiae , 1991, Molecular and cellular biology.

[28]  Curt Wittenberg,et al.  G1-specific cyclins of S. cerevisiae: Cell cycle periodicity, regulation by mating pheromone, and association with the p34 CDC28 protein kinase , 1990, Cell.

[29]  Brenda J. Andrews,et al.  The yeast SWI4 protein contains a motif present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcription , 1989, Nature.

[30]  I. Herskowitz,et al.  Identification of a DNA binding factor involved in cell-cycle control of the yeast HO gene , 1989, Cell.

[31]  L. Breeden,et al.  Similarity between cell-cycle genes of budding yeast and fission yeast and the Notch gene of Drosophila , 1987, Nature.

[32]  Kim Nasmyth,et al.  Cell cycle control of the yeast HO gene: Cis- and Trans-acting regulators , 1987, Cell.

[33]  Kim Nasmyth,et al.  A repetitive DNA sequence that confers cell-cycle START (CDC28)-dependent transcription of the HO gene in yeast , 1985, Cell.