PryJector: A Device for In Situ Visualization of Chemical and Physical Property Distributions on Surfaces Using Projection and Hyperspectral Imaging *,†

Abstract:  Traditional forensic methods that highlight the spatial distribution of properties such as blood and fingerprints have two main disadvantages: they often apply chemicals that may influence further analyses, and they cannot easily be modified to search for new compounds/properties. A new instrument (called PryJector) avoids these problems by dynamically projecting back onto the surface under study spatially distributed information of compounds/properties (chemical images) obtained from multivariate analysis of hyperspectral images. Selectivity to target compounds/properties is ensured by multivariate modeling which makes the instrument much more flexible compared to traditional methods. The functionality of the PryJector is demonstrated in an application related to the detection of counterfeit pharmaceuticals where compounds otherwise indistinguishable to the human eye are made clearly visible by projection of false‐colored chemical images. The PryJector is shown to be a noninvasive and very flexible instrument for highlighting spatial distributions of various compounds/properties.

[1]  Shigeo Abe DrEng.  Pattern Classification , 2001, Springer London.

[2]  Paul N. Newton,et al.  Prevalence and Detection of Counterfeit Pharmaceuticals: A Mini Review , 2008 .

[3]  Claude Roux,et al.  An Examination of the Sequence of Intersecting Lines Using Attenuated Total Reflectance–Fourier Transform Infrared Spectral Imaging * , 2008, Journal of forensic sciences.

[4]  M. Forina,et al.  Multivariate calibration. , 2007, Journal of chromatography. A.

[5]  S T Wu.  Design of a liquid crystal based tunable electrooptic filter. , 1989, Applied optics.

[6]  Patrick J. Treado,et al.  Imaging Spectrometers for Fluorescence and Raman Microscopy: Acousto-Optic and Liquid Crystal Tunable Filters , 1994 .

[7]  Claude Roux,et al.  Visible and near-infrared chemical imaging methods for the analysis of selected forensic samples. , 2005, Talanta.

[8]  Lise Lyngsnes Randeberg,et al.  Skin changes following minor trauma , 2007, Lasers in surgery and medicine.

[9]  Claude Roux,et al.  Applying visible hyperspectral (chemical) imaging to estimate the age of bruises , 2007, Medicine, science, and the law.

[10]  Polarization insensitive tunable liquid-crystal etalon filter , 1991 .

[11]  L. O. Svaasand,et al.  A novel approach to age determination of traumatic injuries by reflectance spectroscopy , 2006, Lasers in surgery and medicine.

[12]  M. Bahner,et al.  Detection of gunshot residues in routine CTs , 2000, International Journal of Legal Medicine.

[13]  Neelam Gupta,et al.  Acousto-optic-tunable-filter-based spectropolarimetric imagers for medical diagnostic applications--instrument design point of view. , 2005, Journal of biomedical optics.

[14]  Fred A. Kruse,et al.  The Spectral Image Processing System (SIPS) - Interactive visualization and analysis of imaging spectrometer data , 1993 .

[15]  A. Berti,et al.  Forensic application of the luminol reaction as a presumptive test for latent blood detection. , 2007, Talanta.

[16]  I. C. Chang.  Tunable Acousto-Optic Filters: An Overview , 1977 .

[17]  P. S. Francis,et al.  Chemiluminescence detection of opium poppy (Papaver somniferum) alkaloids. , 2008, Journal of pharmaceutical and biomedical analysis.

[18]  Hans Brettel,et al.  Multispectral color image capture using a liquid crystal tunable filter , 2002 .

[19]  Mark Tahtouh,et al.  Detection of illicit substances in fingerprints by infrared spectral imaging , 2009, Analytical and bioanalytical chemistry.

[20]  C. Rodrigues,et al.  A new method for collection and identification of gunshot residues from the hands of shooters. , 2003, Journal of forensic sciences.

[21]  Luke N Brewer,et al.  Forensic analysis of bioagents by X-ray and TOF-SIMS hyperspectral imaging. , 2008, Forensic science international.

[22]  Claude Roux,et al.  Forensic applications of chemical imaging: latent fingerprint detection using visible absorption and luminescence. , 2003, Journal of forensic sciences.

[23]  A. Tripathi,et al.  Raman Chemical Imaging of Explosive-Contaminated Fingerprints , 2009, Applied spectroscopy.

[24]  I. Chang.  Acousto-Optic Tunable Filters , 1981 .

[25]  C. Ricci,et al.  Combined Fourier-transform infrared imaging and desorption electrospray-ionization linear ion-trap mass spectrometry for analysis of counterfeit antimalarial tablets , 2007, Analytical and bioanalytical chemistry.

[26]  April Hemmila,et al.  Fourier Transform Infrared Reflectance Spectra of Latent Fingerprints: A Biometric Gauge for the Age of an Individual * , 2008, Journal of forensic sciences.

[27]  S. Baxter,et al.  The Identification of Saliva in Stains in Forensic Casework , 1975, Medicine, science, and the law.

[28]  R. V. Oorschot,et al.  The Use of Polilight® in the Detection of Seminal Fluid, Saliva, and Bloodstains and Comparison with Conventional Chemical‐Based Screening Tests , 2006, Journal of forensic sciences.

[29]  Mark Tahtouh,et al.  The Application of Infrared Chemical Imaging to the Detection and Enhancement of Latent Fingerprints: Method Optimization and Further Findings , 2007, Journal of forensic sciences.

[30]  Patrick J. Treado,et al.  Liquid Crystal Tunable Filter Raman Chemical Imaging , 1996 .

[31]  L. Lytle,et al.  Chemiluminescence in the visualization of forensic bloodstains. , 1978, Journal of forensic sciences.

[32]  C. Lennard,et al.  A further study to investigate the detection and enhancement of latent fingerprints using visible absorption and luminescence chemical imaging. , 2005, Forensic science international.

[33]  Fred A. Kruse,et al.  The Spectral Image Processing System (SIPS): Software for integrated analysis of AVIRIS data , 1992 .

[34]  Claude Roux,et al.  Forensic Analysis of Bicomponent Fibers Using Infrared Chemical Imaging , 2006, Journal of forensic sciences.

[35]  Eunah Lee,et al.  Forensic visualization of foreign matter in human tissue by near‐infrared spectral imaging: Methodology and data mining strategies , 2006, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[36]  Julianne Wolfe,et al.  Characterization of condom lubricant components using Raman spectroscopy and Raman chemical imaging. , 2003, Journal of forensic sciences.

[37]  T. Caelli,et al.  The Application of Remote Sensing for Detecting Mass Graves: An Experimental Animal Case Study from Costa Rica * , 2009, Journal of forensic sciences.

[38]  R. Zoja,et al.  Detection of gunshot residues on cadaveric skin using sodium rhodizonate and a counterstain , 2006, Biotechnic & Histochemistry.