Optoelectrofluidic enhanced immunoreaction based on optically-induced dynamic AC electroosmosis.

We report a novel optoelectrofluidic immunoreaction system based on electroosmotic flow for enhancing antibody-analyte binding efficiency on a surface-based sensing system. Two conventional indium tin oxide glass slides are assembled to provide a reaction chamber for a tiny volume of sample droplet (∼5 μL), in which the top layer is employed as an antibody-immobilized substrate and the bottom layer acts as a photoconductive layer of an optoelectrofluidic device. Under the application of an AC voltage, an illuminated light pattern on the photoconductive layer causes strong counter-rotating vortices to transport analytes from the bulk solution to the vicinity of the assay spot on the glass substrate. This configuration overcomes the slow immunoreaction problem of a diffusion-based sensing system, resulting in the enhancement of binding efficiency via an optoelectrofluidic method. Furthermore, we investigate the effect of optically-induced dynamic AC electroosmotic flow on optoelectrofluidic enhancement for surface-based immunoreaction with a mathematical simulation study and real experiments using immunoglobulin G (IgG) and anti-IgG. As a result, dynamic light patterns provided better immunoreaction efficiency than static light patterns due to effective mass transport of the target analyte, resulting in an achievement of 2.18-fold enhancement under a growing circular light pattern compared to the passive mode.

[1]  Dieter Stoll,et al.  Protein microarrays: Promising tools for proteomic research , 2003, Proteomics.

[2]  W. Boireau,et al.  Improving immunosensor performances using an acoustic mixer on droplet microarray. , 2010, Biosensors & bioelectronics.

[3]  Hyundoo Hwang,et al.  Dynamic light-activated control of local chemical concentration in a fluid. , 2009, Analytical chemistry.

[4]  Hyundoo Hwang,et al.  Generation and manipulation of droplets in an optoelectrofluidic device integrated with microfluidic channels , 2009 .

[5]  Jaebum Choo,et al.  Optoelectrofluidic sandwich immunoassays for detection of human tumor marker using surface-enhanced Raman scattering. , 2010, Analytical chemistry.

[6]  Guy Voirin,et al.  Three-dimensional microfluidic confinement for efficient sample delivery to biosensor surfaces. application to immunoassays on planar optical waveguides. , 2002, Analytical chemistry.

[7]  W. Deen Analysis Of Transport Phenomena , 1998 .

[8]  Ching-Chou Wu,et al.  A label-free impedimetric DNA sensing chip integrated with AC electroosmotic stirring. , 2013, Biosensors & bioelectronics.

[9]  Seong-Won Nam,et al.  Programmable manipulation of motile cells in optoelectronic tweezers using a grayscale image , 2008 .

[10]  Reinhard Niessner,et al.  Microarrays for the screening of allergen-specific IgE in human serum. , 2003, Analytical chemistry.

[11]  Ajit Sadana,et al.  The binding of antigen by immobilized antibody: Influence of a variable adsorption rate coefficient on external diffusion limited kinetics , 1992 .

[12]  Do-Hyun Lee,et al.  Enhanced discrimination of normal oocytes using optically induced pulling-up dielectrophoretic force. , 2009, Biomicrofluidics.

[13]  Jin Jang,et al.  Interactive manipulation of blood cells using a lens‐integrated liquid crystal display based optoelectronic tweezers system , 2008, Electrophoresis.

[14]  M. H. Gazzah,et al.  Flow Confinement Enhancement of Heterogeneous Immunoassays in Microfluidics , 2015, IEEE Sensors Journal.

[15]  Yoon-Kyoung Cho,et al.  In situ dynamic measurements of the enhanced SERS signal using an optoelectrofluidic SERS platform. , 2011, Lab on a chip.

[16]  Hsien-Chang Chang,et al.  A rapid electrochemical biosensor based on an AC electrokinetics enhanced immuno-reaction. , 2013, The Analyst.

[17]  David T. Okou,et al.  Microarray-based genomic selection for high-throughput resequencing , 2007, Nature Methods.

[18]  Ming C. Wu,et al.  Massively parallel manipulation of single cells and microparticles using optical images , 2005, Nature.

[19]  Jiří Homola,et al.  Biosensor Enhancement Using Grooved Micromixers: Part II, Experimental Studies. , 2015, Analytical chemistry.

[20]  Hyundoo Hwang,et al.  Rapid and selective concentration of microparticles in an optoelectrofluidic platform. , 2009, Lab on a chip.

[21]  Robert J. Messinger,et al.  Making it stick: convection, reaction and diffusion in surface-based biosensors , 2008, Nature Biotechnology.

[22]  E. B. Butler,et al.  Antibody microarray profiling of human prostate cancer sera: Antibody screening and identification of potential biomarkers , 2003, Proteomics.

[23]  S. Chao,et al.  Three dimensional simulation on binding efficiency of immunoassay for a biosensor with applying electrothermal effect , 2008, Heat and Mass Transfer.

[24]  Hyundoo Hwang,et al.  Optoelectrofluidic behavior of metal–polymer hybrid colloidal particles , 2013 .

[25]  Castellanos,et al.  AC Electric-Field-Induced Fluid Flow in Microelectrodes. , 1999, Journal of colloid and interface science.