Investigations on virus production in RSV mammalian tumours.

Summary Six different Rous mammalian tumour lines were studied for the occurrence of virus specific antigens, the presence of the viral genome and the synthesis of infectious and non-infectious particles. Four Rous tumour lines were induced in stu mice presumably free from latent murine leukemia virus (MLV), three of them by a cloned Schmidt-Ruppin strain of Rous sarcoma virus (sr-RSV) belonging to sub-group D, the fourth with an uncloned sr-RSV strain. In addition a Prague(P)-RSV induced mouse tumour line (RVP3) and an sr-RSV induced hamster tumour line (RSH) and sr-RSV induced chicken tumours were likewise investigated. In all mammalian lines, except for RVP3, avian leukosis group specific complement-fixing antigen was found. Tumour specific transplantation antigen was demonstrated in all mammalian lines except RSH, whereas no infectious RSV could be detected in any of the mammalian tumours. Likewise electron microscopy revealed no virus particles in more than 9500 cell sections of RSV-stu tumours, but the viral genome was shown to be present by transfer of the tumour to chickens. In the RVP3 line virus particles were detected resembling typical murine leukemia virus while in the RSH line particles were found morphologically similar to the latent hamster virus described by Bernhard & Tournier (1964). The interpretation and significance of the findings are discussed.

[1]  R. Weiss.  The Host Range of bryan Strain Rous Sarcoma Virus Synthesized in the Absence of Helper Virus , 1969 .

[2]  R. Huebner,et al.  Isolation of Naturally Occurring Viruses of the Murine Leukemia Virus Group in Tissue Culture , 1969, Journal of virology.

[3]  T. Graf,et al.  Evidence for the possible existence of two envelope antigenic determinants and corresponding cell receptors for avian tumor viruses. , 1969, Virology.

[4]  W. Schäfer,et al.  Production of a potent complement-fixing murine leukemia virus-antiserum from the rabbit and its reactions with various types of tissue culture cells. , 1968, Virology.

[5]  M. Pollard,et al.  Wide Distribution of Leukaemia Virus in Strains of Laboratory Mice , 1968, Nature.

[6]  H. Hanafusa,et al.  Further studies on RSV production form transformed cells. , 1968, Virology.

[7]  J. Bader,et al.  Production of Virus by Mammalian Cells Transformed by Rous Sarcoma and Murine Sarcoma Viruses , 1968, Journal of virology.

[8]  H. Bauer,et al.  Group-specific antigen of avian leukosis viruses. Virus specificity and relation to an antigen contained in Rous mammalian tumor cells. , 1967, Virology.

[9]  P. Vogt.  A virus released by "nonproducing" Rous sarcoma cells. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[10]  J. Bubeník,et al.  Induction of tumours in mice with three variants of rous sarcoma virus and studies on the immunobiology of these tumours. , 1967, Folia biologica.

[11]  F. Švec,et al.  Virus production in rat tumors induced by chicken sarcoma virus. , 1966, Journal of the National Cancer Institute.

[12]  J. Hartley,et al.  Tissue culture and serologic studies of mouse leukemia viruses. , 1966, National Cancer Institute monograph.

[13]  J. Trentin,et al.  Virus Particles in Hamster Tumors as Revealed by Electron Microscopy.∗ , 1966, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[14]  A. Dalton,et al.  Presence of particles with the morphology of viruses of the avian leukosis complex in meningeal tumors induced in dogs by Rous sarcoma virus. , 1966, Virology.

[15]  G. de‐Thé,et al.  Structure of a murine leukemia virus after disruption with tween-ether and comparison with two myxoviruses. , 1966, Virology.

[16]  F. Švec,et al.  Pathogenicity for rats of a strain of chicken sarcoma virus. , 1966, Journal of the National Cancer Institute.

[17]  P. Veselý,et al.  Line of hamster cells transformed by the Schmidt-Ruppin strain of Rous virus in vitro. , 1966, Folia biologica.

[18]  J. Svoboda.  Basic aspects of the interaction of oncogenic viruses with heterologous cells. , 1966, International review of experimental pathology.

[19]  R. M. Dougherty,et al.  Virus particles associated with "nonproducer" Rous sarcoma cells. , 1965, Virology.

[20]  R. Coggeshall,et al.  A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY , 1965, The Journal of cell biology.

[21]  P. Vogt,et al.  Avian tumor viruses. , 1965, Advances in virus research.

[22]  P. Veselý,et al.  TUMOUR INDUCTION WITH THE ROUS SARCOMA VIRUS IN HAMSTERS AND PRODUCTION OF INFECTIOUS ROUS SARCOMA VIRUS IN AN HETEROLOGOUS HOST. , 1965, Neoplasma.

[23]  E. Harven.  VIRUS PARTICLES IN THE THYMUS OF CONVENTIONAL AND GERM-FREE MICE. , 1964 .

[24]  J. Svoboda,et al.  FORMATION OF DELAYED TUMOURS IN HAMSTERS INOCULATED WITH ROUS VIRUS AFTER BIRTH AND FINDING OF INFECTIOUS ROUS VIRUS IN INDUCED TUMOUR P1. , 1963, Folia biologica.

[25]  John H. Luft,et al.  IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS , 1961, The Journal of biophysical and biochemical cytology.

[26]  J. Svoboda.  Presence of Chicken Tumour Virus in the Sarcoma of the Adult Rat inoculated after Birth with Rous Sarcoma Tissue , 1960, Nature.

[27]  W. Bernhard.  The detection and study of tumor viruses with the electron microscope. , 1960, Cancer research.

[28]  M. A. Epstein.  Observations on the Rous Virus; Fine Structure and Relation to Cytoplasmic Vacuoles , 1957, British Journal of Cancer.