Mucin histochemistry in colonic polyps and cancer.

Mucins are the predominant glycoproteins found in gastrointestinal epithelia, and their structures differ according to the location in the GI tract and the state of cellular differentiation. Different forms of mucin are secreted in colonic polyps and cancers compared to those found in the normal colon. This paper reviews the methods available to probe mucin structure and the state of knowledge regarding the structures of neoplasia-associated mucins. Many of the assumptions made in the interpretation of classical histochemical stains on tissue sections have recently been questioned, and new insight into mucin structure has been gained from the development of newer methodologies such as lectin histochemistry and immunocytochemistry that use well-defined monoclonal antibodies.

[1]  C. Boland,et al.  Comparison of T-antigen expression in normal, premalignant, and malignant human colonic tissue using lectin and antibody immunohistochemistry. , 1986, Cancer research.

[2]  S. Hakomori,et al.  Differential expression of difucosyl type 2 chain (LeY) defined by monoclonal antibody AH6 in different locations of colonic epithelia, various histological types of colonic polyps, and adenocarcinomas. , 1986, Cancer research.

[3]  D. Podolsky,et al.  Oligosaccharide structures of isolated human colonic mucin species. , 1985, The Journal of biological chemistry.

[4]  D. Podolsky,et al.  Oligosaccharide structures of human colonic mucin. , 1985, The Journal of biological chemistry.

[5]  D. Ahnen,et al.  Binding of lectins to goblet cell mucin in malignant and premalignant colonic epithelium in the CF-1 mouse. , 1985, Gastroenterology.

[6]  V. Reuter,et al.  Peanut lectin-binding sites in polyps of the colon and rectum. Adenomas, hyperplastic polyps, and adenomas with in situ carcinoma. , 1983, Laboratory investigation; a journal of technical methods and pathology.

[7]  P. Burtin,et al.  Immunohistological study of precancerous mucus modification in human distal colonic polyps. , 1983, Cancer research.

[8]  K. Isselbacher,et al.  Composition of human colonic mucin. Selective alteration in inflammatory bowel disease. , 1983, The Journal of clinical investigation.

[9]  C. Boland,et al.  A cancer-associated mucin alteration in benign colonic polyps , 1982 .

[10]  C. Boland,et al.  Alterations in human colonic mucin occurring with cellular differentiation and malignant transformation. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[11]  J. Lamont,et al.  Purification and composition of colonic epithelial mucin. , 1980, Biochimica et Biophysica Acta.

[12]  P. Reid,et al.  Specific techniques for the identification of O‐acylated sialic acids in colonic mucins , 1980, Journal of microscopy.

[13]  Y. S. Kim,et al.  Application of lectins for detection of goblet cell glycoconjugate differences in proximal and distal colon of the rat. , 1980, Laboratory investigation; a journal of technical methods and pathology.

[14]  F. Miller,et al.  Chemical and immunological differences between normal and tumoral colonic mucoprotein antigen , 1975, Nature.

[15]  M. Mcentegart,et al.  Loss of Gastro-intestinal-specific Antigen in Neoplasia , 1962, British medical journal.

[16]  P. Burtin,et al.  Early oncofetal antigenic modifications during rat colonic carcinogenesis. , 1983, Cancer research.

[17]  I. Goldstein,et al.  The lectins: carbohydrate-binding proteins of plants and animals. , 1978, Advances in carbohydrate chemistry and biochemistry.