French Swimwear for Membrane Proteins

Integral membrane proteins (IMPs) represent 20 ± 30% of all proteins and well over 50% of the targets for existing drugs. 2] Native IMPs are embedded in the lipid bilayers of biological membranes. The purification of IMPs requires that they first be rendered water soluble. Once solubilized, IMPs may be TMreconstituted∫ back into lipid bilayers or can be directly characterized in soluble form. For example, both 3D crystal growth and solution NMR spectroscopy require the use of solubilized IMPs. Traditionally, membrane proteins are maintained in soluble form by using detergents, which are able to dissolve lipid bilayers to form water-soluble complexes with both lipids and IMPs (Figure 1). Such complexes of detergent with protein and possibly

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