Activated Human Hageman Factor (XII)*

Summary An improved method for the isolation of highly purified active human Hageman factor has been described. An overall recovery of 25% with a purification of 106 has been achieved. Certain of the physical and chemical characteristics of XII have been elucidated. Selective chemical modification of functional groups and a variety of enzymatic assays have been employed in an effort to shed light on the mechanism of action of this procoagulant.

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