In Vitro Hepatitis B virus suppression of erythropoiesis is dependent on the multiplicity of infection and is reversible with anti‐HBs antibodies

Exposure of human bone marrow mononuclear cells to hepatitis B virus results in the suppression of the in vitro growth of several hematopoietic progenitor cells. We studied the degree of inhibition of erythroid progenitor cells that results as a function of the time of exposure of mononuclear cells to hepatitis B virus and the ratio of virus to mononuclear cells, the multiplicity of infection. With an overnight incubation of mononuclear cells with hepatitis B virus‐containing sera, a multiplicity of infection of greater than one virus per mononuclear cell is required to observe significant inhibition of erythroid colony formation. This multiplicity of infection effect is also observed with purified Dane particles. Exposure of mononuclear cells to an increasing number of Dane particles results in a dose‐dependent suppression of erythroid colony formation with significant inhibition observed with a multiplicity of infection of virus to mononuclear cells as low as 5:1. Murine monoclonal antibodies to HBsAg completely neutralize the hepatitis B virus‐mediated inhibition of CFU‐E while control antibodies do not. Purified HBsAg has no effect on colony formation.

[1]  B. Tennant,et al.  Lymphoid cells in the spleens of woodchuck hepatitis virus-infected woodchucks are a site of active viral replication , 1987, Journal of virology.

[2]  J. Wands,et al.  Correlation of HBV DNA and monoclonal reactivity to HBsAg in serum of patients with HBV infection. , 1986, Journal of virological methods.

[3]  J. Melnick,et al.  Extrachromosomal sequences of hepatitis B virus DNA in peripheral blood mononuclear cells of acquired immune deficiency syndrome patients. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[4]  R. Gale,et al.  In vitro hepatitis B virus infection of human bone marrow cells. , 1986, The Journal of clinical investigation.

[5]  R. Purcell,et al.  Hepadnavirus infection of peripheral blood lymphocytes in vivo: woodchuck and chimpanzee models of viral hepatitis , 1986, Journal of virology.

[6]  J. Melnick,et al.  Hepatitis B virus DNA in mononuclear cells and analysis of cell subsets for the presence of replicative intermediates of viral DNA. , 1986, The Journal of infectious diseases.

[7]  M. Poon,et al.  Hepatitis B virus DNA is enriched in polymorphonuclear leukocytes. , 1985, Blood.

[8]  B. Hahn,et al.  Hepatitis B virus DNA sequences in lymphoid cells from patients with AIDS and AIDS-related complex. , 1985, Science.

[9]  C. Crumpacker,et al.  Isolated antibody to hepatitis B surface antigen and response to hepatitis B vaccination. , 1985, Annals of internal medicine.

[10]  P. Pontisso,et al.  Detection of hepatitis B virus DNA in mononuclear blood cells. , 1984, British medical journal.

[11]  A. Dejean,et al.  Detection of hepatitis B virus DNA in pancreas, kidney and skin of two human carriers of the virus. , 1984, The Journal of general virology.

[12]  A. Haase,et al.  Detection of hepatitis B virus DNA in hepatocytes, bile duct epithelium, and vascular elements by in situ hybridization. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[13]  W. Haseltine,et al.  Hepatitis B virus infection in cultured human lymphoblastoid cells. , 1983, Science.

[14]  R. Lerner,et al.  Chemically synthesized peptides of hepatitis B surface antigen duplicate the d/y specificities and induce subtype-specific antibodies in chimpanzees. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[15]  S. Kent,et al.  Specificity of antibodies elicited by a synthetic peptide having a sequence in common with a fragment of a virus protein--the hepatitis B surface antigen. , 1982, Developments in biological standardization.

[16]  M. Balasegaram,et al.  Hepatitis B virus DNA in liver and white blood cells of patients with hepatoma. , 1983, DNA.

[17]  J. Zeldis,et al.  Aplastic anemia and non-A, non-B hepatitis. , 1983, The American journal of medicine.

[18]  Gordon R. Dreesman,et al.  Antibody to hepatitis B surface antigen after a single inoculation of uncoupled synthetic HBsAg peptides , 1982, Nature.

[19]  G. Minot,et al.  Infectious (Catarrhal) Jaundice; An Attempt to Establish a Clinical Entity , 1923 .