A Head-Mounted Spectacle Frame for the Study of Mouse Lens-Induced Myopia

The mouse model has been widely employed to explore the mysteries of myopia. For now, existing techniques for induction of experimental myopia in mice can be classified into three types: (1) devices directly glued to the fur; (2) devices attached using a combination of glue and sutures; (3) devices attached using a skull-mounted apparatus. These techniques each have its advantages, disadvantages when considering the devices stability, safety, complexity, effectiveness, and so forth. Thus, techniques for myopia induction in mice have yet to be further refined to popularize the applications. In this pilot study, we introduce a new head fixation device named the head-mounted spectacle frame apparatus for the study of mouse lens-induced myopia. Surgical procedures for device attachment were relatively simple and easy to learn in our study. Effective myopia induction was validated by retinoscopy refraction and axial length measurement using optical coherence tomography. In addition, it showed improved compliance and reliable safety when compared to the published methods. The head-mounted spectacle frame apparatus provides a new choice for the study of lens-induced myopia in mouse. It also allows for the use of form deprivation, making it attractive for future experimental mouse myopia trials.

[1]  Jia Qu,et al.  [Selection of animal model in myopia research]. , 2005, [Zhonghua yan ke za zhi] Chinese journal of ophthalmology.

[2]  Zhihua Ding,et al.  Assessment of Full-Eye Response to Osmotic Stress in Mouse Model In Vivo Using Optical Coherence Tomography , 2015, Journal of ophthalmology.

[3]  M. Edwards,et al.  Animal models of myopia. A review. , 2009, Acta ophthalmologica Scandinavica.

[4]  Chen Zhao,et al.  Visualization of the ocular pulse in the anterior chamber of the mouse eye in vivo using phase-sensitive optical coherence tomography , 2014, Journal of biomedical optics.

[5]  Ian G Morgan,et al.  Form deprivation and lens‐induced myopia: are they different? , 2013, Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians.

[6]  B. Holden,et al.  Practical applications to modify and control the development of ametropia , 2014, Eye.

[7]  Machelle T. Pardue,et al.  Head-mounted goggles for murine form deprivation myopia , 2007, Journal of Neuroscience Methods.

[8]  Robert W. Williams,et al.  Measurement of Refractive State and Deprivation Myopia in Two Strains of Mice , 2004, Optometry and vision science : official publication of the American Academy of Optometry.

[9]  Machelle T. Pardue,et al.  Retinal degeneration increases susceptibility to myopia in mice , 2013, Molecular vision.

[10]  M. Pardue,et al.  ON pathway mutations increase susceptibility to form-deprivation myopia. , 2015, Experimental eye research.

[11]  Yimin Shen,et al.  Mouse experimental myopia has features of primate myopia. , 2010, Investigative ophthalmology & visual science.

[12]  Eric P.H. Yap,et al.  Two models of experimental myopia in the mouse , 2008, Vision Research.

[13]  K. Naidoo,et al.  Global cost of correcting vision impairment from uncorrected refractive error. , 2012, Bulletin of the World Health Organization.

[14]  Chang-Tai Xu,et al.  Development of biomedical publications on ametropia research in PubMed from 1845 to 2010: a bibliometric analysis. , 2011, International journal of ophthalmology.

[15]  Jia Qu,et al.  [Dose the mice is ideal animal myopia model?]. , 2008, [Zhonghua yan ke za zhi] Chinese journal of ophthalmology.

[16]  Robert W. Williams,et al.  High susceptibility to experimental myopia in a mouse model with a retinal on pathway defect. , 2008, Investigative ophthalmology & visual science.

[17]  W Neil Charman,et al.  Myopia revisited, 2010–2015 , 2015, Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians.

[18]  Earl L. Smith,et al.  Visual regulation of refractive development: insights from animal studies , 2014, Eye.

[19]  Richard A Stone,et al.  Investigating mechanisms of myopia in mice. , 2013, Experimental eye research.

[20]  P. de la Villa,et al.  Refractive changes induced by form deprivation in the mouse eye. , 2003, Investigative ophthalmology & visual science.

[21]  J. Sivak,et al.  Inducing form-deprivation myopia in fish. , 2005, Investigative ophthalmology & visual science.