Latex agglutination test for detection of Escherichia coli serotype O157

The value of a latex agglutination test (Escherichia coli O157 latex test; Oxoid Ltd.) for rapid presumptive detection of E. coli serotype O157:H7 was determined by laboratory trials and during an outbreak of hemorrhagic colitis. The latex test was found to be a simple, highly efficient and reliable test in detecting E. coli O157:H7 with 100% sensitivity and specificity. It was also found that sorbitol-MacConkey agar cultures were not as useful for food samples as they were for fecal specimens in screening for E. coli O157:H7, but the use of the latex screen was particularly efficient in this setting.

[1]  J. Wells,et al.  Illnesses associated with Escherichia coli O157:H7 infections. A broad clinical spectrum. , 1988, Annals of internal medicine.

[2]  J. Carlson,et al.  A severe outbreak of Escherichia coli O157:H7--associated hemorrhagic colitis in a nursing home. , 1987, The New England journal of medicine.

[3]  M. Doyle,et al.  Isolation of Escherichia coli O157:H7 from retail fresh meats and poultry , 1987, Applied and environmental microbiology.

[4]  A. Borczyk,et al.  False positive identifications of Escherichia coli 0157 in foods , 1987 .

[5]  C. Krishnan,et al.  Laboratory investigation of outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7 , 1987, Journal of clinical microbiology.

[6]  B. Rowe,et al.  HAEMORRHAGIC COLITIS AND VERO-CYTOTOXIN-PRODUCING ESCHERICHIA COLI IN ENGLAND AND WALES , 1987, The Lancet.

[7]  A. Borczyk,et al.  BOVINE RESERVOIR FOR VEROTOXIN-PRODUCING ESCHERICHIA COLI 0157:H7 , 1987, The Lancet.

[8]  R. Tauxe,et al.  ISOLATION OF ESCHERICHIA COLI 0157:H7 FROM DAIRY CATTLE ASSOCIATED WITH TWO CASES OF HAEMOLYTIC URAEMIC SYNDROME , 1986, The Lancet.

[9]  W. Gransden,et al.  Further evidence associating hemolytic uremic syndrome with infection by Verotoxin-producing Escherichia coli O157:H7. , 1986, The Journal of infectious diseases.

[10]  S. Ratnam,et al.  Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis , 1986, Journal of clinical microbiology.

[11]  H. Lior,et al.  The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. , 1985, The Journal of infectious diseases.

[12]  C. Pai,et al.  Sporadic cases of hemorrhagic colitis associated with Escherichia coli O157:H7. Clinical, epidemiologic, and bacteriologic features. , 1984, Annals of internal medicine.

[13]  J. Wells,et al.  Sporadic cases of hemorrhagic colitis associated with Escherichia coli O157:H7. , 1984, Annals of internal medicine.

[14]  B. Robison Evaluation of a fluorogenic assay for detection of Escherichia coli in foods , 1984, Applied and environmental microbiology.

[15]  J. Wells,et al.  Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype , 1983, Journal of clinical microbiology.

[16]  A. Steigerwalt,et al.  Atypical biogroups of Escherichia coli found in clinical specimens and description of Escherichia hermannii sp. nov , 1982, Journal of clinical microbiology.