Investigation of the presence of slime production, VanA gene and antiseptic resistance genes in Staphylococci isolated from bovine mastitis in Algeria

Staphylococcus strains are frequently as- sociated with clinical and subclinical bovine intra-mammary infection. The virulence factors of staphylococcus have not been widely studied in Algeria. The objective of this study was to determine the frequency of slime production, VanA gene and antiseptic resistance genes in staphylococci strains isolated from bovine mas- titis in Algeria. The study examined 35 Staphy- lococci strains obtained from the inflammatory secretion of mammary glands of cows with mastitis. Slime production was determined by detecting the icaA and icaD genes using the polymerase chain reaction (PCR) method and Congo red agar (CRA) method. The presence of qacAB and qac C antiseptic resistance genes and the VanA resistance gene in these isolates was investigated by PCR. The results of the current study revealed that of the 35 Staphylo- cocci isolates, 42.85% (15/35) and 17.14% (6/35) of the isolates harboured the slime production gene by analysing icaA and icaD genes, respec- tively and 71.42% (25/35) by the CRA method. However, VanA and antiseptic resistance genes (qacAB and qac C) were not detected in any of the isolates. Therefore, the majority of Staphylo- coccus strains were capable of producing slime, and the CRA detection rate was higher than the PCR method for the biofilm-producing capac- ity of Staphylococcus strains. Thus, the presence of the ica genes in Staphylococcus strains con- firms its role as a virulence factor in the patho- genesis of bovine mastitis.

[1]  R. Stephan,et al.  Staphylococcus aureus Isolates from Goat and Sheep Milk Seem to Be Closely Related and Differ from Isolates Detected from Bovine Milk , 2016, Front. Microbiol..

[2]  H. Solmaz,et al.  Antibiotic susceptibility and molecular identification of antibiotic resistance genes of staphylococci isolated from bovine mastitis in Algeria. , 2015 .

[3]  T. Ali,et al.  Biofilm formation and biofilm-associated genes assay of Staphylococcus aureus isolated from bovine subclinical mastitis in China. , 2014 .

[4]  B. Taminiau,et al.  Genotypic and phenotypic characterization of methicillin‐resistant Staphylococcus aureus (MRSA) isolated from milk of bovine mastitis , 2013, Letters in applied microbiology.

[5]  D. Khelef,et al.  Subclinical mastitis in cattle in Algeria: frequency of occurrence and bacteriological isolates. , 2013, Journal of the South African Veterinary Association.

[6]  L. F. Zafalon,et al.  Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis , 2013, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology].

[7]  G. Azizi,et al.  Detection of the intercellular adhesion gene cluster (ica) in clinical Staphylococcus aureus isolates , 2013, GMS hygiene and infection control.

[8]  Ö. Aslantaş,et al.  Antimicrobial susceptibility, presence of resistance genes and biofilm formation in coagulase negative staphylococci isolated from subclinical sheep mastitis. , 2012 .

[9]  R. Hussain,et al.  Mastitis and associated histo-pathological consequences in the context of udder morphology. , 2012 .

[10]  E. Seker,et al.  Antibiotic susceptibility of coagulase-negative staphylococci isolated from bovine subclinical mastitis in Turkey. , 2012 .

[11]  B. Taminiau,et al.  Genotypic characterization by polymerase chain reaction of Staphylococcus aureus isolates associated with bovine mastitis. , 2011, Veterinary microbiology.

[12]  A. Bakhrouf,et al.  RESEARCH ARTICLE Open Access Detection of macrolide and disinfectant , 2022 .

[13]  Gao Yun-dong.  Biofilm Formation and Analysis of Associated Genes Involved in Staphylococcus Isolates from Bovine Mastitis , 2011 .

[14]  A. Bakhrouf,et al.  Cell surface hydrophobicity, biofilm formation, adhesives properties and molecular detection of adhesins genes in Staphylococcus aureus associated to dental caries. , 2010, Microbial pathogenesis.

[15]  S. Virdis,et al.  Antibiotic Resistance in Staphylococcus aureus and Coagulase Negative Staphylococci Isolated from Goats with Subclinical Mastitis , 2010, Veterinary medicine international.

[16]  Amita Jain,et al.  Biofilm production, a marker of pathogenic potential of colonizing and commensal staphylococci. , 2009, Journal of microbiological methods.

[17]  Chul Hee Choi,et al.  Higher biofilm formation in multidrug-resistant clinical isolates of Staphylococcus aureus. , 2008, International journal of antimicrobial agents.

[18]  Anil Kumar Singh,et al.  Identification and characterization of a vancomycin-resistant Staphylococcus aureus isolated from Kolkata (South Asia). , 2008, Journal of medical microbiology.

[19]  R. Salehi,et al.  Detection of icaAD Gene and Biofilm Formation in Staphylococcus aureus Isolates from Wound Infections , 2006 .

[20]  T. Fatma,et al.  Detection of biofilm formation among the clinical isolates of Staphylococci: an evaluation of three different screening methods. , 2006, Indian journal of medical microbiology.

[21]  C. Gaskins,et al.  Biofilm production by Staphylococcus aureus associated with intramammary infection. , 2005, Veterinary microbiology.

[22]  K. Venkitanarayanan,et al.  Phenotypic and genotypic characterization of bovine mastitis isolates of Staphylococcus aureus for biofilm formation. , 2003, Veterinary microbiology.

[23]  F. Schmitz,et al.  Distribution of the antiseptic resistance genes qacA, qacB and qacC in 497 methicillin-resistant and -susceptible European isolates of Staphylococcus aureus. , 2001, The Journal of antimicrobial chemotherapy.

[24]  B. Markey.  Clinical Veterinary Microbiology , 1993 .