Light microscopic immunocytochemical demonstration of thyroid-stimulating hormone (TSH) receptors on normal rat thyroid cells.

We present a method for immunohistochemical demonstration of surface-associated thyroid-stimulating hormone (TSH) receptors on direct imprints of rat thyroid follicular cells at the light microscopic level, by use of dinitrophenyl (DNP)-labeled bovine TSH as the primary probe in a DNP-hapten sandwich staining (DHSS) procedure. The light microscopically invisible diaminobenzidine (DAB) product of the enzyme reaction was amplified with a DAB enhancement technique (silver amplification), which yielded a reliable and distinct light microscopically visible cell surface staining. The consistently negative control results after inhibition with TSH receptor-specific reagents provide evidence in support of immunohistochemical localization of surface membrane-associated TSH receptors.

[1]  P. Buckland,et al.  The thyrotropin receptor and its role in Graves’ disease1 , 1985, Journal of endocrinological investigation.

[2]  P. Buckland,et al.  A structure for the porcine TSH receptor , 1985 .

[3]  M. Hewlins,et al.  Non-deleterious dinitrophenyl (DNP) hapten labelling of antibody protein. Preparation and properties of some short-chain DNP imidoesters. , 1984, Journal of immunological methods.

[4]  B. Smith,et al.  A RECEPTOR ASSAY FOR THE MEASUREMENT OF TSH RECEPTOR ANTIBODIES IN UNEXTRACTED SERUM , 1984, Clinical endocrinology.

[5]  B. Jasani,et al.  THE VISUALISATION OF TRACE AMOUNTS OF DIAMINOBENZIDINE (DAB) POLYMER BY A NOVEL GOLD‐SULPHIDE‐SILVER METHOD , 1983 .

[6]  J. Schlessinger,et al.  Direct visualization of membrane clustering and endocytosis of thyrotropin into cultured thyroid cells , 1982, Molecular and Cellular Endocrinology.

[7]  T. Bramley.  Properties of LH/hCG receptors in porcine corpus luteum homogenates and subcellular fractions, and factors influencing the recovery of membrane-bound hormone , 1981, Molecular and Cellular Endocrinology.

[8]  B. Jasani,et al.  Use of monoclonal antihapten antibodies for immunolocalisation of tissue antigens , 1981, Journal of clinical pathology.

[9]  E. Yavin,et al.  Monoclonal antibodies to the thyrotropin receptor: implications for receptor structure and the action of autoantibodies in Graves disease. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[10]  S. Formisano,et al.  Dansylated thyrotropin as a probe of hormone-receptor interactions. , 1979, The Journal of biological chemistry.

[11]  M. Stanislawski,et al.  A quantitative study at the single cell level of immunoglobulin antigenic determinants present on the surface of murine B and T lymphocytes. , 1979, Journal of immunology.

[12]  O. Clark,et al.  Binding of rat and bovine thyrotropin to specific sites in rat thyroid tissue. , 1978, Endocrinology.

[13]  B. Weintraub,et al.  Thyrotropin binding to cultured lymphocytes and thyroid cells. , 1978, Endocrinology.

[14]  G. Schatten,et al.  Adhesion of cells to surfaces coated with polylysine. Applications to electron microscopy , 1975, The Journal of cell biology.

[15]  J. Wolff,et al.  Thyroid-stimulating hormone binding to beef thyroid membranes. Relation to adenylate cyclase activity. , 1974, The Journal of biological chemistry.

[16]  R. Hall,et al.  Binding of thyroid stimulators to thyroid membranes , 1974, FEBS letters.

[17]  B. Verrier,et al.  Thyrotropin-binding properties of isolated thyroid cells and their purified plasma membranes. Relation of thyrotropin-specific binding to adenylate-cyclase activation. , 1974, European journal of biochemistry.

[18]  J. Bourke,et al.  Reversible binding of labelled and non-labelled thyrotrophin by intact thyroid tissue in vitro. , 1972, The Journal of endocrinology.

[19]  L. Sternberger,et al.  THE UNLABELED ANTIBODY ENZYME METHOD OF IMMUNOHISTOCHEMISTRY PREPARATION AND PROPERTIES OF SOLUBLE ANTIGEN-ANTIBODY COMPLEX (HORSERADISH PEROXIDASE-ANTIHORSERADISH PEROXIDASE) AND ITS USE IN IDENTIFICATION OF SPIROCHETES , 1970, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[20]  I. Pastan,et al.  BINDING OF HORMONE TO TISSUE: THE FIRST STEP IN POLYPEPTIDE HORMONE ACTION , 1966, Proceedings of the National Academy of Sciences of the United States of America.

[21]  J. Dellacha,et al.  Histological Localization of Fluorescent Thyroid-stimulating Hormone in Rat Tissues , 1959, Nature.