Structure and Assembly of the Yeast V-ATPase

The yeast V-ATPase belongs to a family of V-type ATPases present in all eucaryotic organisms. In Saccharomyces cerevisiae the V-ATPase is localized to the membrane of the vacuole as well as the Golgi complex and endosomes. The V-ATPase brings about the acidification of these organelles by the transport of protons coupled to the hydrolysis of ATP. In yeast, the V-ATPase is composed of 13 subunits consisting of a catalytic V1 domain of peripherally associated proteins and a proton-translocating V0 domain of integral membrane proteins. The regulatory subunit, Vma13p, was the first V-ATPase subunit to have its crystal structure determined. In addition to proteins forming the functional V-ATPase complex, three ER-localized proteins facilitate the assembly of the V0 subunits following their translation and insertion into the membrane of the ER. Homologues of the Vma21p assembly factor have been identified in many higher eukaryotes supporting a ubiquitous assembly pathway for this important enzyme complex.

[1]  A. Cooper,et al.  Degradation of unassembled Vph1p reveals novel aspects of the yeast ER quality control system , 2000, The EMBO journal.

[2]  J. Baleja,et al.  Cysteine Scanning Mutagenesis of the Noncatalytic Nucleotide Binding Site of the Yeast V-ATPase* , 2000, The Journal of Biological Chemistry.

[3]  B. Haarer,et al.  Fluorescence microscopy methods for yeast. , 1989, Methods in cell biology.

[4]  P. Kane,et al.  Novel Vacuolar H+-ATPase Complexes Resulting from Overproduction of Vma5p and Vma13p* , 2002, The Journal of Biological Chemistry.

[5]  P. Kane,et al.  Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase. , 1990, Science.

[6]  T. Stevens,et al.  Molecular Characterization of the Yeast Vacuolar H+-ATPase Proton Pore* , 2000, The Journal of Biological Chemistry.

[7]  Gunnar von Heijne,et al.  Topology Models for 37 Saccharomyces cerevisiaeMembrane Proteins Based on C-terminal Reporter Fusions and Predictions* , 2003, The Journal of Biological Chemistry.

[8]  W. Wickner.  Yeast vacuoles and membrane fusion pathways , 2002, The EMBO journal.

[9]  T. Stevens,et al.  Structure, function and regulation of the vacuolar (H+)-ATPase. , 1997, Annual review of cell and developmental biology.

[10]  P. Kane,et al.  Biochemical characterization of the yeast vacuolar H(+)-ATPase. , 1989, The Journal of biological chemistry.

[11]  T. Stevens,et al.  VMA8 Encodes a 32-kDa V1 Subunit of the Saccharomyces cerevisiae Vacuolar H+-ATPase Required for Function and Assembly of the Enzyme Complex (*) , 1995, The Journal of Biological Chemistry.

[12]  J. Baleja,et al.  Localization of subunits D, E, and G in the yeast V-ATPase complex using cysteine-mediated cross-linking to subunit B. , 2002, Biochemistry.

[13]  D. Botstein,et al.  The VPH1 gene encodes a 95-kDa integral membrane polypeptide required for in vivo assembly and activity of the yeast vacuolar H(+)-ATPase. , 1992, The Journal of biological chemistry.

[14]  T. Stevens,et al.  The Saccharomyces cerevisiae VMA6 gene encodes the 36-kDa subunit of the vacuolar H(+)-ATPase membrane sector. , 1993, The Journal of biological chemistry.

[15]  K. Pfeiffer,et al.  Identification and Characterization of a Novel 9.2-kDa Membrane Sector-associated Protein of Vacuolar Proton-ATPase from Chromaffin Granules* , 1998, The Journal of Biological Chemistry.

[16]  T. Stevens,et al.  VMA11 and VMA16 Encode Second and Third Proteolipid Subunits of the Saccharomyces cerevisiae Vacuolar Membrane H+-ATPase* , 1997, The Journal of Biological Chemistry.

[17]  P. Kane,et al.  The H Subunit (Vma13p) of the Yeast V-ATPase Inhibits the ATPase Activity of Cytosolic V1 Complexes* , 2000, The Journal of Biological Chemistry.

[18]  M. Futai,et al.  Subunit Rotation of Vacuolar-type Proton Pumping ATPase , 2003, Journal of Biological Chemistry.

[19]  D. Klionsky,et al.  Resolution of Subunit Interactions and Cytoplasmic Subcomplexes of the Yeast Vacuolar Proton-translocating ATPase (*) , 1996, The Journal of Biological Chemistry.

[20]  Y. Eilam,et al.  Calcium homeostasis in yeast cells exposed to high concentrations of calcium Roles of vacuolar H+‐ATPase and cellular ATP , 1993, FEBS Letters.

[21]  T. Stevens,et al.  V1-situated Stalk Subunits of the Yeast Vacuolar Proton-translocating ATPase* , 1997, The Journal of Biological Chemistry.

[22]  Kazuhiko Kinosita,et al.  Direct observation of the rotation of F1-ATPase , 1997, Nature.

[23]  H. Arai,et al.  Topography and subunit stoichiometry of the coated vesicle proton pump. , 1988, The Journal of biological chemistry.

[24]  A. Mayer.  Membrane fusion in eukaryotic cells. , 2002, Annual review of cell and developmental biology.

[25]  T. Stevens,et al.  VMA7 encodes a novel 14-kDa subunit of the Saccharomyces cerevisiae vacuolar H(+)-ATPase complex. , 1994, The Journal of biological chemistry.

[26]  T. Stevens,et al.  The Amino-terminal Domain of the Vacuolar Proton-translocating ATPase a Subunit Controls Targeting and in Vivo Dissociation, and the Carboxyl-terminal Domain Affects Coupling of Proton Transport and ATP Hydrolysis* , 2001, The Journal of Biological Chemistry.

[27]  T. Stevens,et al.  Assembly of the Yeast Vacuolar H+-ATPase Occurs in the Endoplasmic Reticulum and Requires a Vma12p/Vma22p Assembly Complex , 1998, The Journal of cell biology.

[28]  T. Stevens,et al.  VMA13 encodes a 54-kDa vacuolar H(+)-ATPase subunit required for activity but not assembly of the enzyme complex in Saccharomyces cerevisiae. , 1993, The Journal of biological chemistry.

[29]  T. Stevens,et al.  VMA12 is essential for assembly of the vacuolar H(+)-ATPase subunits onto the vacuolar membrane in Saccharomyces cerevisiae. , 1993, The Journal of biological chemistry.

[30]  H. Merzendorfer,et al.  A Novel Insect V-ATPase Subunit M9.7 Is Glycosylated Extensively* , 1999, The Journal of Biological Chemistry.

[31]  T. Stevens,et al.  VMA12 Encodes a Yeast Endoplasmic Reticulum Protein Required for Vacuolar H+-ATPase Assembly* , 1997, The Journal of Biological Chemistry.

[32]  Robert Aggeler,et al.  Mechanism of the F(1)F(0)-type ATP synthase, a biological rotary motor. , 2002, Trends in biochemical sciences.

[33]  T. Nishi,et al.  The First Putative Transmembrane Segment of Subunit c" (Vma16p) of the Yeast V-ATPase Is Not Necessary for Function* , 2003, The Journal of Biological Chemistry.

[34]  B. Taillon,et al.  STV1 gene encodes functional homologue of 95-kDa yeast vacuolar H(+)-ATPase subunit Vph1p. , 1994, The Journal of biological chemistry.

[35]  Matthias Mann,et al.  Trans-complex formation by proteolipid channels in the terminal phase of membrane fusion , 2001, Nature.

[36]  T. Stevens,et al.  Vma22p Is a Novel Endoplasmic Reticulum-associated Protein Required for Assembly of the Yeast Vacuolar H+-ATPase Complex (*) , 1995, The Journal of Biological Chemistry.

[37]  J. Baleja,et al.  Cysteine-directed Cross-linking to Subunit B Suggests That Subunit E Forms Part of the Peripheral Stalk of the Vacuolar H+-ATPase* , 2002, The Journal of Biological Chemistry.

[38]  T. Stevens,et al.  Isolation of vacuolar membrane H(+)-ATPase-deficient yeast mutants; the VMA5 and VMA4 genes are essential for assembly and activity of the vacuolar H(+)-ATPase. , 1993, The Journal of biological chemistry.

[39]  J. Liu,et al.  Characterization of a Temperature-sensitive Yeast Vacuolar ATPase Mutant with Defects in Actin Distribution and Bud Morphology* , 1998, The Journal of Biological Chemistry.

[40]  P. Kane,et al.  Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase , 1990, Molecular and cellular biology.

[41]  M. Finbow,et al.  Evidence that there are two copies of subunit c" in V0 complexes in the vacuolar H+-ATPase. , 2002, The Biochemical journal.

[42]  G. Guidotti,et al.  Regulation of Yeast Ectoapyrase Ynd1p Activity by Activator Subunit Vma13p of Vacuolar H+-ATPase* , 2000, The Journal of Biological Chemistry.