Tomato Yellow Leaf Curl Virus-Is Causes a Novel Disease of Common Bean and Severe Epidemics in Tomato in Spain.

Field surveys were conducted in the autumn of 1997 in the main tomato (Lycopersicon esculentum)-growing regions of southern Spain following a severe tomato yellow leaf curl epidemic in tomato. Tomato yellow leaf curl virus (TYLCV)-Is was found to have spread to all regions and to coexist with TYLCV-Sr, which has been present since 1992. TYLCV-Is was also shown to be the causal agent of bean leaf crumple, a novel disease that has caused severe economic losses in fresh-market common bean (Phaseolus vulgaris) crops of southern Spain since September 1997. The disease was reproduced by infecting beans with cloned TYLCV-Is obtained from infected tomato plants collected in Almería. This is the first report of bean leaf crumple disease and the first report of a geminivirus in bean from Spain.

[1]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[2]  J. Polston,et al.  The Emergence Of Whitefly-Transmitted Geminiviruses in Tomato in the Western Hemisphere. , 1997, Plant disease.

[3]  J. Navas-Castillo,et al.  First Report of Tomato Yellow Leaf Curl Virus-Is in Spain: Coexistence of Two Different Geminiviruses in the Same Epidemic Outbreak. , 1997, Plant disease.

[4]  R. Guevara,et al.  Taino Tomato Mottle Virus, a New Bipartite Geminivirus from Cuba. , 1997, Plant disease.

[5]  Yule Liu,et al.  Evidence that DNA-A of a geminivirus associated with severe cassava mosaic disease in Uganda has arisen by interspecific recombination. , 1997, The Journal of general virology.

[6]  I. Zabalgogeazcoa,et al.  Geminiviruses associated with diseased tomatoes in Cuba , 1996 .

[7]  B. Harrison,et al.  Serological studies on the accumulation and localisation of three tomato leaf curl geminiviruses in resistant and susceptible Lycopersicon species and tomato cultivars , 1996 .

[8]  R. Koenig,et al.  Detection of beet necrotic yellow vein virus strains, variants and mixed infections by examining single-strand conformation polymorphisms of immunocapture RT-PCR products. , 1995, The Journal of general virology.

[9]  C. Fauquet,et al.  Complete nucleotide sequence of the geminivirus tomato yellow leaf curl virus, Thailand isolate. , 1994, The Journal of general virology.

[10]  J. Polston,et al.  The nucleotide sequence of tomato mottle virus, a new geminivirus isolated from tomatoes in Florida. , 1992, The Journal of general virology.

[11]  M. Bendahmane,et al.  Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted monopartite geminivirus. , 1991, Nucleic acids research.

[12]  E. Pichersky,et al.  Tomato yellow leaf curl virus: a whitefly-transmitted geminivirus with a single genomic component. , 1991, Virology.

[13]  L. Willmitzer,et al.  Storage of competent cells for Agrobacterium transformation. , 1988, Nucleic acids research.

[14]  M. Bevan,et al.  Binary Agrobacterium vectors for plant transformation. , 1984, Nucleic acids research.

[15]  A. N. Adams,et al.  Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. , 1977, The Journal of general virology.

[16]  H. Czosnek,et al.  A worldwide survey of tomato yellow leaf curl viruses , 1997, Archives of Virology.

[17]  D. Louro,et al.  First report of tomato yellow leaf curl virus in Portugal. , 1996 .

[18]  R. Rivera-Bustamante,et al.  Detection of TYLCV in Cuba. , 1996 .

[19]  E. Noris,et al.  Molecular characterization of cloned DNA from a tomato yellow leaf curl virus isolate from Sicily. , 1995 .

[20]  D. Bois,et al.  First report of a tomato yellow leaf curl-like geminivirus in the Western Hemisphere. , 1994 .

[21]  S. Cohen,et al.  Complete nucleotide sequence of an infectious clone of a mild isolate of tomato yellow leaf curl virus (TYLCV) , 1994 .

[22]  D. Bois,et al.  Tomato yellow leaf curl geminivirus in Jamaica. , 1994 .

[23]  J. Arnó,et al.  First report of tomato yellow leaf curl virus in Spain. , 1993 .

[24]  E. Pichersky,et al.  Use of the polymerase chain reaction to amplify tomato yellow leaf curl virus DNA from infected plants and viruliferous whiteflies , 1992 .