Adipose tissue engineering with human adipose tissue-derived adult stem cells and a novel porous scaffold.

We investigated the effect of a novel porous scaffold composed with water-soluble poly(L-glutamic acid) (PLGA) and chitosan (CS) on the attachment, proliferation, and adipogenic differentiation of human adipose tissue-derived adult stem cells (ADSCs) in vitro and in vivo. Scanning electron microscope and fluorescent Dil labeling were used to reveal the attachment and growth of ADSCs on scaffolds; cell proliferation was detected by DNA assay. The adipogenic differentiation potential of ADSCs on the scaffolds was assayed by Oil-red O staining and further confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) for adipogenic gene markers (peroxisome proliferator-activated receptor γ2, lipoprotein lipase, fatty acid-binding protein, adiponectin). Cell-seeded constructs exposed to adipogenic medium for 2 weeks in vitro were implanted in severe combined immunodeficient (SCID) mice for 6 weeks. It was shown that ADSCs attached and spread well on scaffolds with good proliferation behaviors and abundance of extracellular matrix deposition. Oil-red O staining and RT-PCR showed adipogenic differentiation potential of ADSCs on scaffolds. Newly formed adipose-like tissue was confirmed in vivo in SCID mice by Oil-red O staining. PLGA/CS porous scaffolds exhibit good compatibility to ADSCs and can be promising biomaterials for adipose tissue engineering.

[1]  N. Selvamurugan,et al.  Chitosan scaffolds containing silicon dioxide and zirconia nano particles for bone tissue engineering. , 2011, International journal of biological macromolecules.

[2]  Kai Gong,et al.  Chitosan/silk fibroin-based tissue-engineered graft seeded with adipose-derived stem cells enhances nerve regeneration in a rat model , 2011, Journal of materials science. Materials in medicine.

[3]  Jingbo Yin,et al.  Porous scaffolds based on cross-linking of poly(L-glutamic acid). , 2011, Macromolecular bioscience.

[4]  C. Chai,et al.  Engineering adipose tissue from uncultured human adipose stromal vascular fraction on collagen matrix and gelatin sponge scaffolds. , 2011, Tissue engineering. Part A.

[5]  C. Trahan,et al.  Stacked Deep Inferior Epigastric Perforator Flap Breast Reconstruction: A Review of 110 Flaps in 55 Cases over 3 Years , 2011, Plastic and reconstructive surgery.

[6]  B N Chichkov,et al.  Adipogenic differentiation of laser-printed 3D tissue grafts consisting of human adipose-derived stem cells , 2011, Biofabrication.

[7]  Jingbo Yin,et al.  Layer-by-layer buildup of poly(L-glutamic acid)/chitosan film for biologically active coating. , 2009, Macromolecular bioscience.

[8]  Xuesi Chen,et al.  Biodegradable interpolyelectrolyte complexes based on methoxy poly(ethylene glycol)-b-poly(alpha,L-glutamic acid) and chitosan. , 2008, Biomacromolecules.

[9]  Peter X Ma,et al.  Biomimetic materials for tissue engineering. , 2008, Advanced drug delivery reviews.

[10]  Yilin Cao,et al.  Repair of cranial bone defects with adipose derived stem cells and coral scaffold in a canine model. , 2007, Biomaterials.

[11]  K. Hemmrich,et al.  Biomaterials for adipose tissue engineering , 2006, Expert review of medical devices.

[12]  K. Yasuda,et al.  In situ regeneration of adipose tissue in rat fat pad by combining a collagen scaffold with gelatin microspheres containing basic fibroblast growth factor. , 2006, Tissue engineering.

[13]  Yu Suk Choi,et al.  Adipogenic differentiation of adipose tissue derived adult stem cells in nude mouse. , 2006, Biochemical and biophysical research communications.

[14]  Stephen F Badylak,et al.  The basement membrane component of biologic scaffolds derived from extracellular matrix. , 2006, Tissue engineering.

[15]  Xiabin Jing,et al.  Synthesis and characterization of RGD peptide grafted poly(ethylene glycol)-b-poly(L-lactide)-b-poly(L-glutamic acid) triblock copolymer. , 2006, Biomacromolecules.

[16]  Seung‐Woo Cho,et al.  Engineering of volume-stable adipose tissues. , 2005, Biomaterials.

[17]  Douglas A Kniss,et al.  Adipose tissue model using three-dimensional cultivation of preadipocytes seeded onto fibrous polymer scaffolds. , 2005, Tissue engineering.

[18]  N. Pallua,et al.  Oxygen consumption in undifferentiated versus differentiated adipogenic mesenchymal precursor cells , 2005, Respiratory Physiology & Neurobiology.

[19]  I. Yannas,et al.  Antigenicity and immunogenicity of collagen. , 2004, Journal of biomedical materials research. Part B, Applied biomaterials.

[20]  M. Neubauer,et al.  Generation of mature fat pads in vitro and in vivo utilizing 3-D long-term culture of 3T3-L1 preadipocytes. , 2004, Experimental cell research.

[21]  B. Klosterhalfen,et al.  Analysis of local complications following explantation of silicone breast implants. , 2004, Breast.

[22]  Antonios G Mikos,et al.  Biomimetic materials for tissue engineering. , 2003, Biomaterials.

[23]  H. Hauner,et al.  Tissue engineering of white adipose tissue using hyaluronic acid-based scaffolds. I: in vitro differentiation of human adipocyte precursor cells on scaffolds. , 2003, Biomaterials.

[24]  Min Zhu,et al.  Human adipose tissue is a source of multipotent stem cells. , 2002, Molecular biology of the cell.

[25]  Chun Xing Li.  Poly(L-glutamic acid)--anticancer drug conjugates. , 2002, Advanced drug delivery reviews.

[26]  C. Patrick,et al.  Long-term implantation of preadipocyte-seeded PLGA scaffolds. , 2002, Tissue engineering.

[27]  Min Jung Song,et al.  Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking. , 2002, Biomaterials.

[28]  N. Pallua,et al.  Influence of different biodegradable carriers on the in vivo behavior of human adipose precursor cells. , 2001, Plastic and reconstructive surgery.

[29]  C. Patrick,et al.  Tissue engineering strategies for adipose tissue repair , 2001, The Anatomical record.

[30]  W. Wilkison,et al.  Adipogenic potential of human adipose derived stromal cells from multiple donors is heterogeneous , 2001, Journal of cellular biochemistry.

[31]  H. Lorenz,et al.  Multilineage cells from human adipose tissue: implications for cell-based therapies. , 2001, Tissue engineering.

[32]  E. Nicodemou-Lena,et al.  De novo adipogenesis in mice at the site of injection of basement membrane and basic fibroblast growth factor. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[33]  Mikaël M. Martino,et al.  Biomimetic materials in tissue engineering , 2010 .