The primary structure of ammodytin L, a myotoxic phospholipase A2 homologue from Vipera ammodytes venom.

A new myotoxic phospholipase A2 homologue, having a serine residue in position 49 instead of highly conserved aspartic acid, was found in the venom of Vipera ammodytes. The primary structure revealed additional mutations in the positions important for enzymatic activity. Tyr28 is exchanged for a histidine and Gly33 for asparagine. These changes render earlier-reported weak enzymatic activity unlikely. The role of this rather abundant venom fraction is apparently in myotoxicity, which was confirmed in the muscle-cell culture from neonatal rats. The muscle-cell culture proved to be a good tool to investigate the effects of various myotoxins on muscle cells.

[1]  J. Gutiérrez,et al.  Isolation of basic myotoxins from Bothrops moojeni and Bothrops atrox snake venoms. , 1990, Toxicon : official journal of the International Society on Toxinology.

[2]  D. Turk,et al.  Primary structure of ammodytoxin C further reveals the toxic site of ammodytoxin. , 1989, Biochimica et biophysica acta.

[3]  T. Miyata,et al.  Purification and amino acid sequence of basic protein I, a lysine-49-phospholipase A2 with low activity, from the venom of Trimeresurus flavoviridis (Habu snake). , 1990, Toxicon : official journal of the International Society on Toxinology.

[4]  J. Gutiérrez,et al.  Antibody neutralization of a myotoxin from the venom of Bothrops asper (terciopelo). , 1987, Toxicon : official journal of the International Society on Toxinology.

[5]  J. Drenth,et al.  A comparison of the crystal structures of phospholipase A2 from bovine pancreas and Crotalus atrox venom. , 1985, The Journal of biological chemistry.

[6]  Lowell V. Sick,et al.  Natural Toxins: Animal, Plant, and Microbial , 1986 .

[7]  D. Kordis,et al.  Sequence of the cDNA coding for ammodytoxin B , 1990, Nucleic Acids Res..

[8]  J. Gutiérrez,et al.  A new muscle damaging toxin, myotoxin II, from the venom of the snake Bothrops asper (terciopelo). , 1989, Toxicon : official journal of the International Society on Toxinology.

[9]  C. Takasaki,et al.  Amino acid sequences of eight phospholipases A2 from the venom of Australian king brown snake, Pseudechis australis. , 1990, Toxicon : official journal of the International Society on Toxinology.

[10]  G. Read.  A surface response analysis of the role of salinity in the development of larval and post larval Macrobrachium petersi (Hilgendorf) , 1986 .

[11]  J. Harris,et al.  Rattlesnake Neurotoxins: Biochemical and Biological Aspects , 1990 .

[12]  P. Butler,et al.  [14] Maleylation of amino groups. , 1972, Methods in enzymology.

[13]  B. Strukelj,et al.  Cloning and nucleotide sequence of a cDNA encoding ammodytoxin A, the most toxic phospholipase A2 from the venom of long-nosed viper (Vipera ammodytes). , 1991, Toxicon : official journal of the International Society on Toxinology.

[14]  F. Gubensek,et al.  Ammodytoxin A, a highly lethal phospholipase A2 from Vipera ammodytes ammodytes venom. , 1985, Biochimica et biophysica acta.

[15]  F. Gubensek,et al.  Neuromuscular and lethal effects of phospholipase A from Vipera ammodytes venom. , 1982, Toxicon : official journal of the International Society on Toxinology.

[16]  N. Oda,et al.  Purification and amino acid sequence of basic protein II, a lysine-49-phospholipase A2 with low activity, from Trimeresurus flavoviridis venom. , 1990, Journal of biochemistry.

[17]  T. Kleinschmidt,et al.  Sequence homology between phospholipase and its inhibitor in snake venom. The primary structure of the inhibitor of vipoxin from the venom of the Bulgarian viper (Vipera ammodytes ammodytes, Serpentes). , 1984, Hoppe-Seyler's Zeitschrift fur physiologische Chemie.

[18]  J. Gutiérrez,et al.  Equine antibodies to Bothrops asper myotoxin II: isolation from polyvalent antivenom and neutralizing ability. , 1990, Toxicon : official journal of the International Society on Toxinology.

[19]  C. Yang,et al.  Do chemical modifications dissociate between the enzymatic and pharmacological activities of beta bungarotoxin and notexin? , 1989, Toxicon : official journal of the International Society on Toxinology.

[20]  J. Gutiérrez,et al.  Isolation and partial characterization of a myotoxin from the venom of the snake Bothrops nummifer. , 1986, Toxicon : official journal of the International Society on Toxinology.

[21]  W. Hol,et al.  Structure of bovine pancreatic phospholipase A2 at 1.7A resolution. , 1981, Journal of molecular biology.

[22]  J. Gené,et al.  Myonecrosis induced in mice by a basic myotoxin isolated from the venom of the snake Bothrops nummifer (jumping viper) from Costa Rica. , 1989, Toxicon : official journal of the International Society on Toxinology.

[23]  H. Verheij,et al.  The role of Asp‐49 and other conserved amino acids in phospholipases A2 and their importance for enzymatic activity , 1989, Journal of cellular biochemistry.

[24]  J. Gutiérrez,et al.  Myotoxin II from Bothrops asper (Terciopelo) venom is a lysine-49 phospholipase A2. , 1991, Archives of biochemistry and biophysics.

[25]  P. Bornstein,et al.  [14] Cleavage at AsnGly bonds with hydroxylamine , 1977 .

[26]  B. Strukelj,et al.  Nucleotide sequence of a cDNA encoding ammodytin L. , 1990, Nucleic acids research.

[27]  R. Heinrikson,et al.  Amino acid sequence of phospholipase A2-alpha from the venom of Crotalus adamanteus. A new classification of phospholipases A2 based upon structural determinants. , 1977, The Journal of biological chemistry.

[28]  R. Komel,et al.  Amino acid sequence of ammodytoxin C as deduced from cDNA. , 1989, Nucleic acids research.

[29]  Y. Nomura,et al.  Pertussis toxin-sensitive GTP-binding proteins may regulate phospholipase A2 in response to thrombin in rabbit platelets. , 1989, Archives of biochemistry and biophysics.

[30]  L. Rodrigues-Simioni,et al.  Fractionation of Bothrops jararacussu snake venom: partial chemical characterization and biological activity of bothropstoxin. , 1988, Toxicon : official journal of the International Society on Toxinology.

[31]  V. Turk,et al.  Amino-acid sequence of ammodytoxin B partially reveals the location of the site of toxicity of ammodytoxins. , 1986, Biological chemistry Hoppe-Seyler.

[32]  A. Bieber,et al.  The effects of myotoxin from midget faded rattlesnake (Crotalus viridis concolor) venom on neonatal rat myotubes in cell culture. , 1986, Toxicon : official journal of the International Society on Toxinology.

[33]  R. Lewis,et al.  A complete amino acid sequence for the basic subunit of crotoxin. , 1986, Archives of biochemistry and biophysics.

[34]  H. Verheij,et al.  The role of aspartic acid-49 in the active site of phospholipase A2. A site-specific mutagenesis study of porcine pancreatic phospholipase A2 and the rationale of the enzymatic activity of [lysine49]phospholipase A2 from Agkistrodon piscivorus piscivorus' venom. , 1988, European journal of biochemistry.