The crystal structure of the gene targeting homing endonuclease I-SceI reveals the origins of its target site specificity.

[1]  A. Zanghellini,et al.  A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells. , 2003, Nucleic acids research.

[2]  Monique Turmel,et al.  Flexible DNA target site recognition by divergent homing endonuclease isoschizomers I-CreI and I-MsoI. , 2003, Journal of molecular biology.

[3]  Dana Carroll,et al.  Enhancing Gene Targeting with Designed Zinc Finger Nucleases , 2003, Science.

[4]  David Baltimore,et al.  Chimeric Nucleases Stimulate Gene Targeting in Human Cells , 2003, Science.

[5]  B. Stoddard,et al.  Catalytic mechanisms of restriction and homing endonucleases. , 2002, Biochemistry.

[6]  J. Joly,et al.  I-SceI meganuclease mediates highly efficient transgenesis in fish , 2002, Mechanisms of Development.

[7]  B. Stoddard,et al.  Design, activity, and structure of a highly specific artificial endonuclease. , 2002, Molecular cell.

[8]  F. Quiocho,et al.  Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence , 2002, Nature Structural Biology.

[9]  B. Stoddard,et al.  Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility. , 2001, Nucleic acids research.

[10]  A. Waldman,et al.  Capture of DNA sequences at double-strand breaks in mammalian chromosomes. , 2001, Genetics.

[11]  A. Spassky,et al.  Chemical Probing Shows That the Intron-encoded Endonuclease I-SceI Distorts DNA through Binding in Monomeric Form to Its Homing Site* , 2001, The Journal of Biological Chemistry.

[12]  Nicholas M. Luscombe,et al.  Amino acid?base interactions: a three-dimensional analysis of protein?DNA interactions at an atomic level , 2001, Nucleic Acids Res..

[13]  Barry L. Stoddard,et al.  The homing endonuclease I-CreI uses three metals, one of which is shared between the two active sites , 2001, Nature Structural Biology.

[14]  A. Pingoud,et al.  Identification of Asp218 and Asp326 as the principal Mg2+ binding ligands of the homing endonuclease PI-SceI. , 2000, Biochemistry.

[15]  K Morikawa,et al.  Crystal structure of an archaeal intein-encoded homing endonuclease PI-PfuI. , 2000, Journal of molecular biology.

[16]  F. S. Gimble.  Invasion of a multitude of genetic niches by mobile endonuclease genes. , 2000, FEMS microbiology letters.

[17]  A. Pingoud,et al.  The monomeric homing endonuclease PI‐SceI has two catalytic centres for cleavage of the two strands of its DNA substrate , 1999, The EMBO journal.

[18]  Cécile Fairhead,et al.  Mitochondrial DNA repairs double-strand breaks in yeast chromosomes , 1999, Nature.

[19]  B. Matthews,et al.  Type II restriction endonucleases: structural, functional and evolutionary relationships. , 1999, Current opinion in chemical biology.

[20]  N. Perrimon,et al.  I-SceI endonuclease, a new tool for studying DNA double-strand break repair mechanisms in Drosophila. , 1999, Genetics.

[21]  M. Belfort,et al.  Crystal structure of the thermostable archaeal intron-encoded endonuclease I-DmoI. , 1999, Journal of molecular biology.

[22]  F. Quiocho,et al.  Identification of Lys-403 in the PI-SceI Homing Endonuclease as Part of a Symmetric Catalytic Center* , 1998, The Journal of Biological Chemistry.

[23]  B. Stoddard,et al.  DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI. , 1998, Molecular cell.

[24]  R J Read,et al.  Crystallography & NMR system: A new software suite for macromolecular structure determination. , 1998, Acta crystallographica. Section D, Biological crystallography.

[25]  M. Jasin,et al.  Analysis of Gene Targeting and Intrachromosomal Homologous Recombination Stimulated by Genomic Double-Strand Breaks in Mouse Embryonic Stem Cells , 1998, Molecular and Cellular Biology.

[26]  F. Jaisser,et al.  I-SceI-Induced Gene Replacement at a Natural Locus in Embryonic Stem Cells , 1998, Molecular and Cellular Biology.

[27]  R. Monnat,et al.  Genetic analysis of the Chlamydomonas reinhardtii I-CreI mobile intron homing system in Escherichia coli. , 1997, Genetics.

[28]  R J Roberts,et al.  Homing endonucleases: keeping the house in order. , 1997, Nucleic acids research.

[29]  B. Stoddard,et al.  The structure of I-Crel, a Group I intron-encoded homing endonuclease , 1997, Nature Structural Biology.

[30]  F. Quiocho,et al.  Crystal Structure of PI-SceI, a Homing Endonuclease with Protein Splicing Activity , 1997, Cell.

[31]  R M Esnouf,et al.  An extensively modified version of MolScript that includes greatly enhanced coloring capabilities. , 1997, Journal of molecular graphics & modelling.

[32]  B. Dujon,et al.  Induction of homologous recombination in mammalian chromosomes by using the I-SceI system of Saccharomyces cerevisiae , 1995, Molecular and cellular biology.

[33]  Frederick S. Gimble,et al.  Substitutions in Conserved Dodecapeptide Motifs That Uncouple the DNA Binding and DNA Cleavage Activities of PI-SceI Endonuclease (*) , 1995, The Journal of Biological Chemistry.

[34]  P. Rouet,et al.  Introduction of double-strand breaks into the genome of mouse cells by expression of a rare-cutting endonuclease. , 1994, Molecular and cellular biology.

[35]  E A Merritt,et al.  Raster3D Version 2.0. A program for photorealistic molecular graphics. , 1994, Acta crystallographica. Section D, Biological crystallography.

[36]  C. Jacq,et al.  I-Sce III an intron-encoded DNA endonuclease from yeast mitochondria. Asymmetrical DNA binding properties and cleavage reaction. , 1993, Nucleic acids research.

[37]  B. Dujon,et al.  Asymmetrical recognition and activity of the I‐SceI endonuclease on its site and on intron‐exon junctions. , 1993, The EMBO journal.

[38]  P. Kraulis.  A program to produce both detailed and schematic plots of protein structures , 1991 .

[39]  J. Zou,et al.  Improved methods for building protein models in electron density maps and the location of errors in these models. , 1991, Acta crystallographica. Section A, Foundations of crystallography.

[40]  G. Schmitz,et al.  Cleavage of yeast and bacteriophage T7 genomes at a single site using the rare cutter endonuclease I-Sce I , 1991, Nucleic Acids Res..

[41]  B. Dujon,et al.  Purification and characterization of the in vitro activity of I-Sce I, a novel and highly specific endonuclease encoded by a group I intron. , 1990, Nucleic acids research.

[42]  B. Dujon,et al.  Recognition and cleavage site of the intron-encoded omega transposase. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[43]  R Lavery,et al.  The definition of generalized helicoidal parameters and of axis curvature for irregular nucleic acids. , 1988, Journal of biomolecular structure & dynamics.

[44]  B. Dujon,et al.  Universal code equivalent of a yeast mitochondrial intron reading frame is expressed into E. coli as a specific double strand endonuclease , 1986, Cell.

[45]  Bernard Dujon,et al.  An intron-encoded protein is active in a gene conversion process that spreads an intron into a mitochondrial gene , 1985, Cell.

[46]  H. Puchta.  Use of I-Sce I to induce DNA double-strand breaks in Nicotiana. , 1999, Methods in molecular biology.

[47]  Z. Otwinowski,et al.  [20] Processing of X-ray diffraction data collected in oscillation mode. , 1997, Methods in enzymology.

[48]  G. Bricogne,et al.  [27] Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods. , 1997, Methods in enzymology.

[49]  P. Rouet,et al.  Double-strand breaks at the target locus stimulate gene targeting in embryonic stem cells. , 1995, Nucleic acids research.

[50]  P. Sharp,et al.  Positive genetic selection for gene disruption in mammalian cells by homologous recombination. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[51]  M. Capecchi.  The new mouse genetics: altering the genome by gene targeting. , 1989, Trends in genetics : TIG.