Fluorescence responses from nitrogen plant stress in 4 Fraunhofer band regions

The potential of solar Fraunhofer line features centered at 532, 607, 677 and 745nm for tracking changes in plant canopy chlorophyll content and photosynthetic capacity was studied. Excitation wavelengths similar to full sun light were considered. Canopy changes were tested experimentally by monitoring treatments of plant stress due to nitrogen application rate in corn. Corn leaves were obtained from field plots that were given different nitrogen application rates at 20, 50, 100, and 150% of optimal N in 2001. The data infers that leaves in plant canopies that have the greatest photosynthetic performance potential can be identified. Information collected in Fraunhofer regions compared favorably with data taken by laser induced fluorescence excitation and detection methods in peak emission areas. The technique may be useful in projecting what can be expected if a space-born interferometer type sensor can be developed for capturing plant canopy fluorescence.

[1]  Hartmut K. Lichtenthaler,et al.  The Role of Chlorophyll Fluorescence in The Detection of Stress Conditions in Plants , 1988 .

[2]  J. McMurtrey,et al.  Laser-induced fluorescence of green plants. 2: LIF caused by nutrient deficiencies in corn. , 1984, Applied optics.

[3]  Arnold F. Theisen,et al.  A Passive Method for Detecting Vegetation Stress from Orbit: Chlorophyll Fluorescence Spectra from Fraunhofer Lines , 2000 .

[4]  G. Krause,et al.  Chlorophyll Fluorescence and Photosynthesis: The Basics , 1991 .

[5]  Hartmut K. Lichtenthaler,et al.  Decrease of the chlorophyll fluorescence ratio F690/F730 during greening and development of leaves , 1990, Radiation and environmental biophysics.

[6]  Moon S. Kim,et al.  Distinguishing nitrogen fertilization levels in field corn (Zea mays L.) with actively induced fluorescence and passive reflectance measurements , 1994 .

[7]  G. Carter,et al.  Detection of solar-excited chlorophyll a fluorescence and leaf photosynthetic capacity using a fraunhofer line radiometer , 1996 .

[8]  J. McMurtrey,et al.  Laser-induced fluorescence of green plants. 1: A technique for the remote detection of plant stress and species differentiation. , 1984, Applied optics.

[9]  S. Bose,et al.  CHLOROPHYLL FLUORESCENCE IN GREEN PLANTS AND ENERGY TRANSFER PATHWAYS IN PHOTOSYNTHESIS , 1982 .

[10]  Moon S. Kim,et al.  Identification of the pigment responsible for the blue fluorescence band in the laser induced fluorescence (LIF) spectra of green plants, and the potential use of this band in remotely estimating rates of photosynthesis , 1991 .

[11]  L.A. Corp,et al.  Optimal fluorescence excitation wavelengths for detection of stress in vegetation , 1996, IGARSS '96. 1996 International Geoscience and Remote Sensing Symposium.

[12]  J. A. Plascyk.  The MK II Fraunhofer Line Discriminator (FLD-II) for Airborne and Orbital Remote Sensing of Solar-Stimulated Luminescence , 1975 .

[13]  Arnold F. Theisen,et al.  A passive two-band sensor of sunlight-excited plant fluorescence , 1999 .

[14]  Hartmut K. Lichtenthaler,et al.  Leaf chlorophyll fluorescence corrected for re-absorption by means of absorption and reflectance measurements , 1998 .