Sidelobe decline in single-photon 4Pi microscopy by Toraldo rings.

We demonstrate theoretically the feasibility of single-photon 4Pi-confocal microscopy. By inserting a pair of properly designed multi-ring phase-only pupil filters in the illumination path of a 4Pi microscope the height of the sidelobes of the point spread function substantially reduced, so that there is no ambiguity in the 3D image. Then, an axial resolution up to four times higher than that of single-photon confocal microscope can be effectively achieved.

[1]  Pedro Andrés,et al.  Tunable axial superresolution by annular binary filters. Application to confocal microscopy , 1995 .

[2]  Stefan W. Hell,et al.  Fundamental improvement of resolution with a 4Pi-confocal fluorescence microscope using two-photon excitation , 1992 .

[3]  S W Hell,et al.  Coherent use of opposing lenses for axial resolution increase. II. Power and limitation of nonlinear image restoration. , 2001, Journal of the Optical Society of America. A, Optics, image science, and vision.

[4]  Amparo Pons,et al.  Axial apodization in 4Pi-confocal microscopy by annular binary filters. , 2002, Journal of the Optical Society of America. A, Optics, image science, and vision.

[5]  S W Hell,et al.  Coherent use of opposing lenses for axial resolution increase in fluorescence microscopy. I. Comparative study of concepts. , 2001, Journal of the Optical Society of America. A, Optics, image science, and vision.

[6]  S. Hell,et al.  Properties of a 4Pi confocal fluorescence microscope , 1992 .

[7]  E. Wolf,et al.  Electromagnetic diffraction in optical systems, II. Structure of the image field in an aplanatic system , 1959, Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences.

[8]  Stefan W. Hell,et al.  Single sharp spot in fluorescence microscopy of two opposing lenses. , 2001 .

[9]  J. Pawley,et al.  Handbook of Biological Confocal Microscopy , 1990, Springer US.

[10]  Jim Swoger,et al.  Tailoring the axial shape of the point spread function using the Toraldo concept. , 2002, Optics express.