An investigation of the potential application of chitosan/aloe-based membranes for regenerative medicine.

A significant number of therapeutics derived from natural polymers and plants have been developed to replace or to be used in conjunction with existing dressing products. The use of the therapeutic properties of aloe vera could be very useful in the creation of active wound dressing materials. The present work was undertaken to examine issues concerning structural features, topography, enzymatic degradation behavior, antibacterial activity and cellular response of chitosan/aloe vera-based membranes. The chitosan/aloe vera-based membranes that were developed displayed satisfactory degradation, roughness, wettability and mechanical properties. A higher antibacterial potency was displayed by the blended membranes. Moreover, in vitro assays demonstrated that these blended membranes have good cell compatibility with primary human dermal fibroblasts. The chitosan/aloe vera-based membranes might be promising wound dressing materials.

[1]  Xiu-lian Chang,et al.  Water-soluble polysaccharides isolated from skin juice, gel juice and flower of Aloe vera Miller , 2011 .

[2]  J. Desbrières,et al.  An infrared investigation in relation with chitin and chitosan characterization , 2001 .

[3]  R. Reis,et al.  Plasma surface modification of chitosan membranes: characterization and preliminary cell response studies. , 2008, Macromolecular bioscience.

[4]  Rui L Reis,et al.  Potential applications of natural origin polymer-based systems in soft tissue regeneration , 2010, Critical reviews in biotechnology.

[5]  M. Wink.  Medicinal Plants: A Source of Anti-Parasitic Secondary Metabolites , 2012, Molecules.

[6]  D. G. Armstrong,et al.  Wound Microbiology and Associated Approaches to Wound Management , 2001, Clinical Microbiology Reviews.

[7]  Tomaz Velnar,et al.  The Wound Healing Process: An Overview of the Cellular and Molecular Mechanisms , 2009, The Journal of international medical research.

[8]  R. Reis,et al.  Soft Constructs for Skin Tissue Engineering , 2012 .

[9]  B. Escalante,et al.  Antiinflammatory activity of extracts from Aloe vera gel. , 1996, Journal of ethnopharmacology.

[10]  M. Chan-Park,et al.  Effect of argon-plasma treatment on proliferation of human-skin-derived fibroblast on chitosan membrane in vitro. , 2005, Journal of biomedical materials research. Part A.

[11]  Xiaojun Ma,et al.  The enzymatic degradation and swelling properties of chitosan matrices with different degrees of N-acetylation. , 2005, Carbohydrate research.

[12]  G. Sajithlal,et al.  Influence of aloe vera on the healing of dermal wounds in diabetic rats. , 1998, Journal of ethnopharmacology.

[13]  B D Boyan,et al.  Role of material surfaces in regulating bone and cartilage cell response. , 1996, Biomaterials.

[14]  R. Reis,et al.  Effect of chitosan membrane surface modification via plasma induced polymerization on the adhesion of osteoblast-like cells , 2007 .

[15]  Jarupa Viyoch,et al.  Effects of the blended fibroin/aloe gel film on wound healing in streptozotocin-induced diabetic rats , 2012, Biomedical materials.

[16]  S. P. C. Filho,et al.  Características e propriedades de quitosanas purificadas nas formas neutra, acetato e cloridrato , 2001 .

[17]  K. Eshun,et al.  Aloe Vera: A Valuable Ingredient for the Food, Pharmaceutical and Cosmetic Industries—A Review , 2004, Critical reviews in food science and nutrition.

[18]  S. Arunkumar,et al.  Analysis of phytochemical constituents and antimicrobial activities of Aloe vera L. against clinical pathogens. , 2009 .

[19]  Josias H. Hamman,et al.  Composition and Applications of Aloe vera Leaf Gel , 2008, Molecules.

[20]  R. Reis,et al.  Morphology and miscibility of chitosan/soy protein blended membranes , 2007 .

[21]  Walter Steurbaut,et al.  Chitosan as antimicrobial agent: applications and mode of action. , 2003, Biomacromolecules.

[22]  Jiangfeng Zhu,et al.  Study on antimicrobial activity of chitosan with different molecular weights , 2003 .

[23]  Frank Devlieghere,et al.  Chitosan: antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables , 2004 .

[24]  M. Rinaudo,et al.  Chitin and chitosan: Properties and applications , 2006 .

[25]  Anubhav Tripathi,et al.  Viscoelastic response of human skin to low magnitude physiologically relevant shear. , 2008, Journal of biomechanics.

[26]  S. Petrulytė.  Advanced textile materials and biopolymers in wound management. , 2008, Danish medical bulletin.

[27]  J. Boateng,et al.  Wound healing dressings and drug delivery systems: a review. , 2008, Journal of pharmaceutical sciences.

[28]  F. Silver,et al.  Viscoelastic properties of human skin and processed dermis , 2001, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[29]  J. van Staden,et al.  Antimicrobial, anti-inflammatory and mutagenic investigation of the South African tree aloe (Aloe barberae). , 2009, Journal of ethnopharmacology.

[30]  Moon Suk Kim,et al.  Gradient polymer surfaces for biomedical applications , 2008 .

[31]  J. Henry.  Clinical diagnosis and management by laboratory methods , 1979 .

[32]  R. Pandey,et al.  Antibacterial Activities of Crude Extract of Aloe barbadensis to Clinically Isolated Bacterial Pathogens , 2010, Applied biochemistry and biotechnology.

[33]  Eugene Khor,et al.  Chitosan-alginate PEC membrane as a wound dressing: Assessment of incisional wound healing. , 2002, Journal of biomedical materials research.

[34]  S. Verma,et al.  International Journal of Biological & Medical Research Aloe Vera Their Chemicals Composition and Applications: a Review a B , 2022 .

[35]  J. Mano.  Viscoelastic properties of chitosan with different hydration degrees as studied by dynamic mechanical analysis. , 2008, Macromolecular bioscience.

[36]  H. S. Azevedo,et al.  Chitosan scaffolds incorporating lysozyme into CaP coatings produced by a biomimetic route: a novel concept for tissue engineering combining a self-regulated degradation system with in situ pore formation. , 2009, Acta biomaterialia.

[37]  Ho-Sun Lim,et al.  Advances in Chitosan Material and its Hybrid Derivatives: A Review , 2009 .

[38]  D. Chou,et al.  Clinical Diagnosis and Management by Laboratory Methods , 2001 .

[39]  I. Tizard,et al.  Activation of a mouse macrophage cell line by acemannan: the major carbohydrate fraction from Aloe vera gel. , 1996, Immunopharmacology.

[40]  R L Reis,et al.  Effect of crosslinking in chitosan/aloe vera-based membranes for biomedical applications. , 2013, Carbohydrate polymers.

[41]  D. K. Owens,et al.  Estimation of the surface free energy of polymers , 1969 .