Spectral Doppler optical coherence tomography imaging of localized ischemic stroke in a mouse model.

We report the use of spectral Doppler optical coherence tomography imaging (SDOCTI) for quantitative evaluation of dynamic blood circulation before and after a localized ischemic stroke in a mouse model. Rose Bengal photodynamic therapy (PDT) is used as a noninvasive means for inducing localized ischemia in cortical microvasculature of the mouse. Fast, repeated Doppler optical coherence tomography scans across vessels of interest are performed to record flow dynamic information with high temporal resolution. Doppler-angle-independent flow indices are used to quantify vascular conditions before and after the induced ischemia by the photocoagulation of PDT. The higher (or lower) flow resistive indices are associated with higher (or lower) resistance states that are confirmed by laser speckle flow index maps (of laser speckle imaging). Our in vivo experiments shows that SDOCTI can provide complementary quantified flow information that is an alternative to blood volume measurement, and can be used as a means for cortical microvasculature imaging well suited for small animal studies.

[1]  Ruikang K. Wang,et al.  Doppler optical micro-angiography for volumetric imaging of vascular perfusion in vivo. , 2009, Optics express.

[2]  Bernard Choi,et al.  Spectral Doppler imaging of micro-vasculature response to laser irradiation , 2009, BiOS.

[3]  Zhongping Chen,et al.  Phase-resolved optical coherence tomography and optical Doppler tomography for imaging blood flow in human skin with fast scanning speed and high velocity sensitivity. , 2000, Optics letters.

[4]  Bernard Choi,et al.  Linear response range characterization and in vivo application of laser speckle imaging of blood flow dynamics. , 2006, Journal of biomedical optics.

[5]  Michael Schroeter,et al.  Non-invasive induction of focal cerebral ischemia in mice by photothrombosis of cortical microvessels: characterization of inflammatory responses , 2002, Journal of Neuroscience Methods.

[6]  D. Kleinfeld,et al.  Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[7]  S. Thomas Carmichael,et al.  Rodent models of focal stroke: Size, mechanism, and purpose , 2005, NeuroRX.

[8]  A W Toga,et al.  Mapping functional activity in rodent cortex using optical intrinsic signals. , 1994, Cerebral cortex.

[9]  Lingfeng Yu,et al.  Doppler variance imaging for three-dimensional retina and choroid angiography. , 2010, Journal of biomedical optics.

[10]  J. Schuman,et al.  Optical coherence tomography. , 2000, Science.

[11]  Arthur W. Toga,et al.  Imaging Optical Reflectance in Rodent Barrel and Forelimb Sensory Cortex , 1994, NeuroImage.

[12]  M. Moskowitz,et al.  Dynamic Imaging of Cerebral Blood Flow Using Laser Speckle , 2001, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[13]  Huihua Kenny Chiang,et al.  Imaging pulsatile retinal blood flow in human eye. , 2008, Journal of biomedical optics.

[14]  D. Ts'o,et al.  Cortical functional architecture and local coupling between neuronal activity and the microcirculation revealed by in vivo high-resolution optical imaging of intrinsic signals. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[15]  S. Starkman,et al.  Neuroprotective agents for the treatment of acute ischemic stroke , 2003, Current neurology and neuroscience reports.

[16]  D. C. Miller,et al.  A photothrombotic model of small early ischemic infarcts in the rat brain with histologic and MRI correlation. , 2001, Journal of pharmacological and toxicological methods.

[17]  Zhongping Chen,et al.  Optical Doppler tomographic imaging of fluid flow velocity in highly scattering media. , 1997, Optics letters.

[18]  Tae-Sun Kim,et al.  Photochemically induced cerebral ischemia in a mouse model. , 2007, Surgical neurology.

[19]  Katsuhiko Suzuki,et al.  Characterization of inflammatory responses to eccentric exercise in humans. , 2005, Exercise immunology review.

[20]  J M Rubin,et al.  Relationship between the resistive index and vascular compliance and resistance. , 1999, Radiology.