PCR to predict risk of airborne disease.

Plant, animal and human diseases spread by microscopic airborne particles have had major economic and social impacts during history. Special air-sampling devices have been used to collect such particles since the 19th century but it has often been impossible to identify them accurately. Exciting new opportunities to combine air sampling with quantitative PCR to identify and count these particles are reviewed, using crop pathogen examples. These methods can be used to predict the risk of unexpected outbreaks of airborne diseases by identifying increases in pathogen inoculum or genetic changes in pathogen populations that render control ineffective. The predictions can provide guidance to policymakers, health professionals or the agricultural industry for the development of strategies to minimise the risk of severe pandemics.

[1]  M. Garbelotto,et al.  Detection and Quantification of Airborne Conidia of Fusarium circinatum, the Causal Agent of Pine Pitch Canker, from Two California Sites by Using a Real-Time PCR Approach Combined with a Simple Spore Trapping Method , 2004, Applied and Environmental Microbiology.

[2]  C. Gaillard,et al.  Real-time quantitative PCR for the design of lentiviral vector analytical assays , 2005, Gene Therapy.

[3]  B. Thomma,et al.  Recent developments in pathogen detection arrays: implications for fungal plant pathogens and use in practice. , 2005, Phytopathology.

[4]  J. Campbell,et al.  Cheese worker's hypersensitivity pneumonitis. , 1983, The American review of respiratory disease.

[5]  Robert A. Samson,et al.  Food mycology : a multifaceted approach to fungi and food , 2007 .

[6]  H. A. McCartney,et al.  Dispersal of foliar plant pathogens: mechanisms, gradients and spatial patterns , 2006 .

[7]  T. Michailides,et al.  Quantification of airborne spores of Monilinia fructicola in stone fruit orchards of California using real-time PCR , 2007, European Journal of Plant Pathology.

[8]  J. M. Steed,et al.  Patterns of ascospore release in relation to phoma stem canker epidemiology in England (Leptosphaeria maculans) and Poland (Leptosphaeria biglobosa) , 2005, European Journal of Plant Pathology.

[9]  P. Bowyer,et al.  Septoria on Cereals: A Study of Pathosystems , 1999 .

[10]  JonathanH. West,et al.  The air spora , 2006 .

[11]  S A Bustin,et al.  Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. , 2002, Journal of molecular endocrinology.

[12]  Linda D. Stetzenbach,et al.  Introduction to Aerobiology , 2007 .

[13]  S. Alexandersen,et al.  New directions: Airborne transmission of foot-and-mouth disease virus , 2004 .

[14]  David S Gregory.  Pertussis: a disease affecting all ages. , 2006, American family physician.

[15]  B. Cooke,et al.  The Epidemiology of Plant Diseases , 1998, Springer Netherlands.

[16]  S. Bonini,et al.  Allergenic pollen and pollen allergy in Europe , 2007, Allergy.

[17]  James K. M. Brown,et al.  Aerial Dispersal of Pathogens on the Global and Continental Scales and Its Impact on Plant Disease , 2002, Science.

[18]  N. Wratten,et al.  Two weather‐based models for predicting the onset of seasonal release of ascospores of Leptosphaeria maculans or L. biglobosa , 2007 .

[19]  M. Metzker.  Emerging technologies in DNA sequencing. , 2005, Genome research.

[20]  Grace Chang,et al.  Molecular diagnosis of medical viruses. , 2007, Current issues in molecular biology.

[21]  Elaine Ward,et al.  Plant pathogen diagnostics : immunological and nucleic acid-based approaches , 2004 .

[22]  Peter Gladders,et al.  Range and severity of a plant disease increased by global warming , 2008, Journal of The Royal Society Interface.

[23]  M. Shaw,et al.  Application of real-time and multiplex polymerase chain reaction assays to study leaf blotch epidemics in barley. , 2007, Phytopathology.

[24]  J. West,et al.  Role of Ascospores in Further Spread of QoI-Resistant Cytochrome b Alleles (G143A) in Field Populations of Mycosphaerella graminicola. , 2005, Phytopathology.

[25]  C. Vallavieille-Pope,et al.  Wind-dispersed diseases , 1998 .

[26]  M. Milgroom,et al.  Intercontinental population structure of the chestnut blight fungus, Cryphonectria parasitica , 1996 .

[27]  J. West,et al.  Dispersal of fungal spores through the air , 2007 .

[28]  R. Kennedy,et al.  A New Method To Monitor Airborne Inoculum of the Fungal Plant Pathogens Mycosphaerella brassicicola andBotrytis cinerea , 2000, Applied and Environmental Microbiology.

[29]  Raymond Tellier,et al.  Review of Aerosol Transmission of Influenza A Virus , 2006, Emerging infectious diseases.

[30]  Mikhail A. Semenov,et al.  Modelling impacts of climate change on wheat yields in England and Wales: assessing drought risks , 2005 .

[31]  J. Russo,et al.  The Integrated Aerobiology Modeling System applied to the spread of soybean rust into the Ohio River valley during September 2006 , 2007 .

[32]  A. Mills,et al.  Manual of environmental microbiology. , 2007 .

[33]  A. Awad.  Airborne dust, bacteria, actinomycetes and fungi at a flourmill , 2007 .

[34]  The potato and the pathogen , 1995 .

[35]  N. Ward,et al.  Policy Framing and Learning the Lessons from the UK's Foot and Mouth Disease Crisis , 2004 .

[36]  N. Pace.  A molecular view of microbial diversity and the biosphere. , 1997, Science.

[37]  M. Deloge-Abarkan,et al.  Detection of airborne Legionella while showering using liquid impingement and fluorescent in situ hybridization (FISH). , 2007, Journal of environmental monitoring : JEM.

[38]  J. M. Hirst,et al.  The summer air-spora at Rothamsted in 1952. , 1957, Journal of general microbiology.

[39]  B. Nelson,et al.  Sclerotinia sclerotiorum (Lib.) de Bary: biology and molecular traits of a cosmopolitan pathogen. , 2006, Molecular plant pathology.

[40]  P. Esker,et al.  Spatiotemporal Description of Epidemics Caused by Phoma ligulicola in Tasmanian Pyrethrum Fields. , 2005, Phytopathology.

[41]  S. Makino,et al.  Application of the real-time PCR for the detection of airborne microbial pathogens in reference to the anthrax spores. , 2003, Journal of microbiological methods.

[42]  C. Ribble,et al.  Reporting of suspect cases of foot-and-mouth-disease during the 2001 epidemic in the UK, and the herd sensitivity and herd specificity of clinical diagnosis. , 2007, Preventive veterinary medicine.

[43]  P. Brook.  Stimulation of Ascospore Release in Venturia inaequalis by Far Red Light , 1969, Nature.

[44]  D. Volokhov,et al.  A multiplex polymerase chain reaction microarray assay to detect bioterror pathogens in blood. , 2005, The Journal of molecular diagnostics : JMD.

[45]  S. Savary,et al.  Spatiotemporal relationships between disease development and airborne inoculum in unmanaged and managed Botrytis leaf blight epidemics. , 2008, Phytopathology.

[46]  X. Xu,et al.  A dynamic model forecasting infection of pear leaves by conidia of Venturia nashicola and its evaluation in unsprayed orchards , 2007, European Journal of Plant Pathology.

[47]  N. Boonham,et al.  Using real-time PCR to discriminate and quantify the closely related wheat pathogens Oculimacula yallundae and Oculimacula acuformis , 2005 .

[48]  Cécile Viboud,et al.  Lessons from the past: Familial aggregation analysis of fatal pandemic influenza (Spanish flu) in Iceland in 1918 , 2008, Proceedings of the National Academy of Sciences.

[49]  Jennifer S. Falacy,et al.  Detection of Erysiphe necator in Air Samples Using the Polymerase Chain Reaction and Species-Specific Primers. , 2007, Phytopathology.

[50]  G. Agrawal,et al.  Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes. , 2007, Current genomics.

[51]  E. Domingo,et al.  Foot-and-mouth disease virus. , 2002, Comparative immunology, microbiology and infectious diseases.

[52]  D. Schmale,et al.  Spatial Patterns of Viable Spore Deposition of Gibberella zeae in Wheat Fields. , 2005, Phytopathology.

[53]  J. West,et al.  Effects of timing of Leptosphaeria maculans ascospore release and fungicide regime on phoma leaf spot and phoma stem canker development on winter oilseed rape (Brassica napus) in southern England , 2002 .

[54]  J. Ristaino,et al.  PCR amplification of the Irish potato famine pathogen from historic specimens , 2001, Nature.

[55]  Gabriele Neumann,et al.  Human infection with highly pathogenic H5N1 influenza virus , 2008, The Lancet.

[56]  Mark Hernandez,et al.  Incorporating polymerase chain reaction-based identification, population characterization, and quantification of microorganisms into aerosol science: A review , 2006, Atmospheric Environment.

[57]  D. Aylor.  The Aerobiology of Apple Scab. , 1998, Plant disease.

[58]  Tania Nolan,et al.  Quantification of mRNA using real-time RT-PCR , 2006, Nature Protocols.

[59]  A. McCartney,et al.  Detection of airborne fungal spores sampled by rotating-arm and Hirst-type spore traps using polymerase chain reaction assays , 2002 .

[60]  R. Frederick,et al.  Advances in molecular-based diagnostics in meeting crop biosecurity and phytosanitary issues. , 2003, Annual review of phytopathology.

[61]  C. Ingold.  Active liberation of reproductive units in terrestrial fungi , 1999 .

[62]  S. Welham,et al.  Predicting light leaf spot (Pyrenopeziza brassicae) risk on winter oilseed rape (Brassica napus) in England and Wales, using survey, weather and crop information , 2004 .

[63]  T. Paulitz,et al.  Spatial Distribution of Venturia inaequalis Airborne Ascospores in Orchards. , 2002, Phytopathology.

[64]  C. Barnes,et al.  Detection and identification of four common rust pathogens of cereals and grasses using real-time polymerase chain reaction. , 2007, Phytopathology.

[65]  Elaine Ward,et al.  Methods for Integrated Air Sampling and DNA Analysis for Detection of Airborne Fungal Spores , 2001, Applied and Environmental Microbiology.

[66]  S. Viljanen-Rollinson,et al.  Monitoring long-distance spore dispersal by wind - a review , 2007 .