Escherichia coli contains plasmids coding for heat-stable b, other enterotoxins, and antibiotic resistance

Plasmid DNAs obtained from 18 Escherichia coli isolates that hybridized with the heat-stable b (ST-b) enterotoxin gene probe were examined by Southern blot analysis for genes coding for heat-labile, ST-a, and ST-b enterotoxins with specific radiolabeled DNA probes. Four E. coli isolates contained plasmids coding for both heat-labile and ST-b enterotoxins, and one isolate contained a plasmid coding for ST-a and ST-b. Five of 11 isolates of antibiotic-resistant enterotoxigenic E. coli isolates containing ST-b-coding DNA transferred a plasmid coding for both antibiotic resistance and ST-b to E. coli K-12, suggesting that the widespread use of antibiotics could increase the distribution of genes coding for ST-b.

[1]  David N. Taylor,et al.  Identification by DNA hybridization of enterotoxigenic Escherichia coli in a longitudinal study of villages in Thailand. , 1985, The Journal of infectious diseases.

[2]  P. Echeverria,et al.  Prevalence of heat-stable II enterotoxigenic Escherichia coli in pigs, water, and people at farms in Thailand as determined by DNA hybridization , 1984, Journal of clinical microbiology.

[3]  S. Moseley,et al.  Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enterotoxigenic Escherichia coli heat-stable toxin II producers , 1983, Infection and immunity.

[4]  H. Heyneker,et al.  Nucleotide sequence of the gene for heat-stable enterotoxin II of Escherichia coli , 1983, Infection and immunity.

[5]  P. Echeverria,et al.  Identification of enterotoxigenic Escherichia coli by colony hybridization using three enterotoxin gene probes. , 1982, The Journal of infectious diseases.

[6]  R. Yolken,et al.  A Two-Year Study of Bacterial, Viral, and Parasitic Agents Associated with Diarrhea in Rural Bangladesh , 1980, The Journal of infectious diseases.

[7]  R. Black,et al.  USE OF ANTISERA FOR IDENTIFICATION OF ENTEROTOXIGENIC ESCHERICHIA COLI , 1980, The Lancet.

[8]  R. Lathe,et al.  Cell-free synthesis of enterotoxin of E. coli from a cloned gene , 1980, Nature.

[9]  H. Moon,et al.  Prevalence of pilus antigens, enterotoxin types, and enteropathogenicity among K88-negative enterotoxigenic Escherichia coli from neonatal pigs. , 1980, Infection and immunity.

[10]  H. Birnboim,et al.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA. , 1979, Nucleic acids research.

[11]  S. Falkow,et al.  Cistrons encoding Escherichia coli heat-labile toxin , 1979, Journal of bacteriology.

[12]  F. Heffron,et al.  The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1 , 1979, Nature.

[13]  P. Echeverria,et al.  ANTIMICROBIAL RESISTANCE AND ENTEROTOXIN PRODUCTION AMONG ISOLATES OF ESCHERICHIA COLI IN THE FAR EAST , 1978, The Lancet.

[14]  S. Falkow,et al.  Characterization of an Escherichia coli plasmid encoding for synthesis of heat-labile toxin: molecular cloning of the toxin determinant , 1978, Infection and immunity.

[15]  R. Bywater,et al.  Biological evaluation of a methanol-soluble, heat-stable Escherichia coli enterotoxin in infant mice, pigs, rabbits, and calves , 1978, Infection and immunity.

[16]  W. Maas,et al.  Naturally occurring plasmid carrying genes for enterotoxin production and drug resistance. , 1977, Science.

[17]  J. Shapiro,et al.  DNA: Insertion Elements, Plasmids, and Episomes. , 1977 .

[18]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[19]  T. Maniatis,et al.  Nucleotide sequence of the rightward operator of phage lambda. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[20]  D. Sack,et al.  Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture , 1975, Infection and immunity.

[21]  R. Sack,et al.  Human diarrheal disease caused by enterotoxigenic Escherichia coli. , 1975, Annual review of microbiology.

[22]  S. Falkow,et al.  The enterotoxin plasmids of Escherichia coli. , 1974, The Journal of infectious diseases.

[23]  A. Dean,et al.  Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. , 1972, The Journal of infectious diseases.

[24]  C. Gyles,et al.  A study of Escherichia coli strains isolated from pigs with gastro-intestinal disease. , 1971, Canadian journal of comparative medicine : Revue canadienne de medecine comparee.

[25]  H. Moon,et al.  Development of resistance with age by swine intestine to effects of enteropathogenic Escherichia coli. , 1970, The Journal of infectious diseases.

[26]  H. Smith,et al.  Observations by the ligated intestinal segment and oral inoculation methods on Escherichia coli infections in pigs, calves, lambs and rabbits. , 1967, The Journal of pathology and bacteriology.

[27]  A. Bauer,et al.  Antibiotic susceptibility testing by a standardized single disk method. , 1966, American journal of clinical pathology.