Staurosporine inhibits tyrosine-specific protein kinase activity of Rous sarcoma virus transforming protein p60.

The disclosure is of a novel closure for air evacuated tubular containers and comprises a tubular body having flexible, elastic sidewalls, an open end, a closed end formed by a cannula-pierceable, flexible, elastic end wall having a concavo-convex configuration in cross-section and a flange disposed radially about the periphery of the open end. The novel closure structure takes advantage of the vacuum force in the container to maintain a gas-proof, hermetic seal, reduces the thickness required of the closure to maintain the hermetic seal and is easier to assemble in an air evacuated container. The disclosure is also of a novel method of assembling the closure of the invention in an air evacuated tubular container.

[1]  A. Ullrich,et al.  The complete primary structure of protein kinase C--the major phorbol ester receptor. , 1986, Science.

[2]  J. Ishida,et al.  A specific inhibitor for tyrosine protein kinase from Pseudomonas. , 1986, The Journal of antibiotics.

[3]  T. Tamaoki,et al.  Staurosporine, a potent inhibitor of phospholipid/Ca++dependent protein kinase. , 1986, Biochemical and biophysical research communications.

[4]  H. Iba,et al.  Lack of induction of neuroretinal cell proliferation by Rous sarcoma virus variants that carry the c-src gene , 1985, Molecular and cellular biology.

[5]  F. Cross,et al.  Low level of cellular protein phosphorylation by nontransforming overproduced p60c-src , 1985, Molecular and cellular biology.

[6]  M. Perry,et al.  P1,P4-Di(adenosine-5')tetraphosphate inhibits phosphorylation of immunoglobulin G by Rous sarcoma virus pp60src. , 1983, The Journal of biological chemistry.

[7]  H. Hanafusa,et al.  Role of p60src kinase activity in the induction of neuroretinal cell proliferation by rous sarcoma virus , 1982, Journal of virology.

[8]  I. Pastan,et al.  Inhibition of the transformation-specific kinase in ASV-transformed cells by N-α-tosyl-L-lysyl chloromethyl ketone , 1979, Cell.

[9]  S Omura,et al.  A new alkaloid AM-2282 OF Streptomyces origin. Taxonomy, fermentation, isolation and preliminary characterization. , 1977, The Journal of antibiotics.

[10]  E. Rapaport,et al.  Presence of diadenosine 5',5''' -P1, P4-tetraphosphate (Ap4A) in mamalian cells in levels varying widely with proliferative activity of the tissue: a possible positive "pleiotypic activator". , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[11]  M. Barbacid,et al.  A human oncogene formed by the fusion of truncated tropomyosin and protein tyrosine kinase sequences , 1986, Nature.

[12]  M. Imoto,et al.  Studies on a new epidermal growth factor-receptor kinase inhibitor, erbstatin, produced by MH435-hF3. , 1986, The Journal of antibiotics.