Effect of exposure to sublethal concentrations of sodium cyanide on the carbohydrate metabolism of the Indian Major Carp Labeo rohita (Hamilton, 1822)

Experiments were designed to study in-vivo effects of sodium cyanide on biochemical endpoints in the freshwater fish Labeo rohita. Fish were exposed to two sublethal concentrations (0.106 and 0.064mg/L) for a period of 15 days.  Levels of glycogen, pyruvate, lactate and the enzymatic activities of lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), glucose-6-phosphate dehydrogenase (G6PDH), phosphorylase, alkaline phosphatase (ALP), acid phosphatase (AcP) were assessed in different tissues (liver, muscle and gills). Result indicated a steady decrease in glycogen, pyruvate, SDH, ALP and AcP activity with a concomitant increase in the lactate, phosphorylase, LDH and G6PD activity in all selected tissues. The alterations in all the above biochemical parameters were significantly (p<0.05) time and dose dependent. In all the above parameters, liver pointing out the intensity of cyanide intoxication compare to muscle and gills. Study revealed change in the metabolic energy by means of altered metabolic profile of the fish. Further, these observations indicated that even sublethal concentrations of sodium cyanide might not be fully devoid of deleterious influence on metabolism in L. rohita.

[1]  A. E. Greenberg,et al.  Standard Methods for the Examination of Water and Wastewater seventh edition , 2013 .

[2]  E. N. Ogamba,et al.  Effect of Diazinon on Acid and Alkaline Phosphatase Activities in Plasma and Organs of Clarias gariepinus , 2011 .

[3]  E. A. Caxton-martins,et al.  Neurotoxicity of cassava: Mode of cell death in the visual relay centres of adult wistar rats , 2010 .

[4]  R. Bhattacharya,et al.  Cyanide-induced changes in the levels of neurotransmitters in discrete brain regions of rats and their response to oral treatment with alpha-ketoglutarate. , 2010, Indian journal of experimental biology.

[5]  R. C. Rocha-e-Silva,et al.  Cyanide toxicity and interference with diet selection in quail (Coturnix coturnix). , 2010, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[6]  B. Hosetti,et al.  Assessment of acute toxicity of sodium cyanide to a freshwater teleost, Labeo rohita (Ham). , 2010 .

[7]  S. Ceyhun,et al.  In vitro and in vivo effects of some pesticides on glucose-6-phosphate dehydrogenase enzyme activity from rainbow trout (Oncorhynchus mykiss) erythrocytes , 2009 .

[8]  R. Bhattacharya,et al.  Acute toxicity of some synthetic cyanogens in rats and their response to oral treatment with alpha-ketoglutarate. , 2009, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[9]  B. Rees,et al.  Exercise- and hypoxia-induced anaerobic metabolism and recovery: a student laboratory exercise using teleost fish. , 2009, Advances in physiology education.

[10]  G. Begum.  Enzymes as Biomarkers of Cypermethrin Toxicity: Response of Clarias batrachus Tissues ATPase and Glycogen Phosphorylase as a Function of Exposure and Recovery at Sublethal Level , 2009, Toxicology mechanisms and methods.

[11]  R. B. Walker,et al.  Cyanide-induced free radical production and lipid peroxidation in rat brain homogenate is reduced by aspirin , 2000, Metabolic Brain Disease.

[12]  S. Neelagund,et al.  Free cyanide-induced biochemical changes in nitrogen metabolism of the Indian major carp Cirrhinus mrigala , 2007, Journal of basic and clinical physiology and pharmacology.

[13]  M. Balasubramanian,et al.  Impact of Distillery Effluent on Carbohydrate Metabolism of Freshwater Fish, Cyprinus carpio , 2005, Ecotoxicology.

[14]  B. Das,et al.  Toxicity of cypermethrin in Labeo rohita fingerlings: biochemical, enzymatic and haematological consequences. , 2003, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[15]  E. Vicaut,et al.  Value of lactic acidosis in the assessment of the severity of acute cyanide poisoning , 2002, Critical care medicine.

[16]  R. Panneerselvam,et al.  Effect of sodium molybdate on carbohydrate metabolizing enzymes in alloxan-induced diabetic rats. , 2002, The Journal of nutritional biochemistry.

[17]  A. Osagie,et al.  Differential effects of chronic cyanide intoxication on heart, lung and pancreatic tissues. , 2000, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[18]  A. Zwaan,et al.  Sulfide and cyanide induced mortality and anaerobic metabolism in the arcid blood clam Scapharca inaequiv alvis , 1993 .

[19]  D. Penney,et al.  Cardiovascular, metabolic and neurologic effects of carbon monoxide and cyanide in the rat. , 1992, Toxicology letters.

[20]  K. R. Ramana Rao,et al.  Significance of glucose-6-phosphate dehydrogenase activity in tissues of penaeid prawn,Metapenaeus monoceros (Fabricius) under acute kelthane exposure , 1991, Bulletin of environmental contamination and toxicology.

[21]  R. Braithwaite,et al.  The metabolic effects of fatal cyanide poisoning. , 1989, Postgraduate medical journal.

[22]  M. Bollen,et al.  The hepatic glycogenolysis induced by reversible ischaemia or KCN is exclusively catalysed by phosphorylase a. , 1988, The Biochemical journal.

[23]  A. Heath.  Water Pollution and Fish Physiology , 1987 .

[24]  J. Sneyd,et al.  The action of anoxia and cyanide on glycogen breakdown in the liver of the gsd/gsd rat. , 1984, European journal of biochemistry.

[25]  S. Govindappa,et al.  Electrophoretic characteristics of subcellular components & their relation to enzyme activities in the amphibian muscle fibre. , 1965, Indian journal of experimental biology.

[26]  Hans Ulrich Bergmeyer,et al.  Methods of Enzymatic Analysis , 2019 .

[27]  W. Huckabee.  Relationship of pyruvate and lactate during anaerobic metabolism. V. Coronary adequacy. , 1961, The American journal of physiology.

[28]  J. H. Roe,et al.  The determination of glycogen in liver and muscle by use of anthrone reagent. , 1956, The Journal of biological chemistry.

[29]  E. Sutherland.  [26] Polysaccharide phosphorylase, liver: Polysaccharide + P ⥂ G-1-P☆ , 1955 .

[30]  E. King,et al.  Estimation of Plasma Phosphatase by Determination of Hydrolysed Phenol with Amino-antipyrine , 1954, Journal of clinical pathology.

[31]  T. Friedemann,et al.  PYRUVIC ACID I. COLLECTION OF BLOOD FOR THE DETERMINATION OF PYRUVIC AND LACTIC ACIDS , 1942 .

[32]  C. H. Fiske,et al.  THE COLORIMETRIC DETERMINATION OF PHOSPHORUS , 1925 .