16S Ribosomal DNA Terminal Restriction Fragment Pattern Analysis of Bacterial Communities in Feces of Rats Fed Lactobacillus acidophilus NCFM

ABSTRACT 16S ribosomal DNA terminal restriction fragment patterns from rat fecal samples were analyzed to track the dynamics ofLactobacillus acidophilus NCFM and discern bacterial populations that changed during feeding with NCFM. Lactobacillus johnsonii and Ruminococcus flavefaciens were tentatively identified as such bacterial populations. The presence ofL. johnsonii was confirmed by isolation from feces.

[1]  P. Saxman,et al.  Terminal Restriction Fragment Length Polymorphism Analysis Program, a Web-Based Research Tool for Microbial Community Analysis , 2000, Applied and Environmental Microbiology.

[2]  B. B. Jensen,et al.  Changes in Bacterial Community Structure in the Colon of Pigs Fed Different Experimental Diets and after Infection with Brachyspira hyodysenteriae , 2000, Applied and Environmental Microbiology.

[3]  H. Harmsen,et al.  Analysis of the Fecal Microflora of Human Subjects Consuming a Probiotic Product Containing Lactobacillus rhamnosusDR20 , 2000, Applied and Environmental Microbiology.

[4]  L. Kerkhof,et al.  Horizontal Heterogeneity of Denitrifying Bacterial Communities in Marine Sediments by Terminal Restriction Fragment Length Polymorphism Analysis , 2000, Applied and Environmental Microbiology.

[5]  Katharine G. Field,et al.  Identification of Nonpoint Sources of Fecal Pollution in Coastal Waters by Using Host-Specific 16S Ribosomal DNA Genetic Markers from Fecal Anaerobes , 2000, Applied and Environmental Microbiology.

[6]  C. Kitts,et al.  Isolating PCR-quality DNA from human feces with a soil DNA kit. , 2000, BioTechniques.

[7]  W. Liesack,et al.  Spatial Changes in the Bacterial Community Structure along a Vertical Oxygen Gradient in Flooded Paddy Soil Cores , 2000, Applied and Environmental Microbiology.

[8]  T. Akao.  Influence of various bile acids on the metabolism of glycyrrhizin and glycyrrhetic acid by Ruminococcus sp. PO1-3 of human intestinal bacteria. , 1999, Biological & pharmaceutical bulletin.

[9]  G. B. Fogel,et al.  Prokaryotic Genome Size and SSU rDNA Copy Number: Estimation of Microbial Relative Abundance from a Mixed Population , 1999, Microbial Ecology.

[10]  C. Rao,et al.  Prevention of colonic preneoplastic lesions by the probiotic Lactobacillus acidophilus NCFMTM in F344 rats. , 1999, International journal of oncology.

[11]  Gerwin C. Raangs,et al.  Variations of Bacterial Populations in Human Feces Measured by Fluorescent In Situ Hybridization with Group-Specific 16S rRNA-Targeted Oligonucleotide Probes , 1998, Applied and Environmental Microbiology.

[12]  Hans H. Cheng,et al.  Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA , 1997, Applied and environmental microbiology.

[13]  W. Wenzhi,et al.  Molecular analysis of the composition of the bifidobacterial and lactobacillus microflora of humans , 1996, Applied and environmental microbiology.

[14]  K. Wilson,et al.  Human colonic biota studied by ribosomal DNA sequence analysis , 1996, Applied and environmental microbiology.

[15]  C. Brunk,et al.  A computer analysis of primer and probe hybridization potential with bacterial small-subunit rRNA sequences , 1996, Applied and environmental microbiology.

[16]  C. Brunk,et al.  Molecular technique for rapid identification of mycobacteria , 1996, Journal of clinical microbiology.

[17]  C. Brunk,et al.  A molecular technique for identification of bacteria using small subunit ribosomal RNA sequences. , 1994, BioTechniques.

[18]  B. Decaris,et al.  Strain characterization, genome size and plasmid content in the Lactobacillus acidophilus group (Hansen and Mocquot). , 1993, The Journal of applied bacteriology.

[19]  Rebekka R. E. Artz,et al.  University of Groningen Association of marine archaea with the digestive tracts of two marine fish species , 2017 .

[20]  James R. Cole,et al.  The RDP (Ribosomal Database Project) continues , 2000, Nucleic Acids Res..

[21]  L. Forney,et al.  Determination of the microbial diversity of anaerobic-aerobic activated sludge by a novel molecular biological technique , 1998 .

[22]  Larry J. Forney,et al.  Beginning a molecular analysis of the eukaryal community in activated sludge , 1998 .

[23]  Christopher L. Kitts,et al.  Terminal restriction fragment patterns (TRFPs), a rapid, PCR-based method for the comparison of complex bacterial communities , 1998 .