Comparison of tritium-labeled steroids for progesterone receptor assay in human endometrium.

Tritium-labeled steroids [progesterone, medroxyprogesterone acetate, ORG 2058 [16α-ethyl-21-hydroxy-19-nor-4-pregnene-3, 20-dione), R5020 (17, 21-dimethyl-19-norpregna-4, 9-diene-3, 20-dione), norethindrone and R1881 (17β-hydroxy-17α-methylestra-4, 9, 11-trien-3-one)] were evaluated and discussed for progesterone receptor assay in the cytosol of human uterine endometrium. Each labeled steroid bound to the receptor proteins for progesterone, possibly estrogen or androgen, and occasionally the proteins in the globulin fraction. It is suggested that it is better not to use a tritium-labeled steroid with the same steroid as a competitor in order to get a non-specific binder for the measurement of progesterone receptor assay. The value of the dissociation constant was between 10-9M and 10-10M whatever tritium-labeled or unlabeled steroid was used.

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