Treatment of a simulated wastewater amended with a chiral pharmaceuticals mixture by an aerobic granular sludge sequencing batch reactor

An aerobic granular sludge-sequencing batch reactor (AGS-SBR) was fed for 28-days with a simulated wastewater containing a mixture of chiral pharmaceuticals (CPs) (alprenolol, bisoprolol, metoprolol, propranolol, venlafaxine, salbutamol, fluoxetine and norfluoxetine), each at 1.3 μg L−1. AGS-SBR exhibited the highest removal efficiency for norfluoxetine, with preferential removal of the (R)-enantiomer indicating that biological-mediated processes occurred. For all other CPs, removal was non-enantioselective, occurring through biosorption onto AGS. A gradual decline of CPs removal was observed, probably related to the decrease of AGS adsorption capacity. Moreover, chemical oxygen demand (COD) content in the bulk liquid after anaerobic feeding increased, and P-release dropped, probably because the polyphosphate-accumulating organism's activity was affected. Nitrification was also affected as indicated by the ammonium effluent concentration increase. Moreover, CPs exposure promoted AGS disintegration, with decreasing granule size. After stopping CPs feeding, the AGS started to recover its compact structure, and the system returned its normal performance concerning COD- and P-removal. N-removal seemed to be a more sensitive process, as while the ammonium levels were fully restored at the end of operation, nitrite reduction was only partially restored. Results provide useful information on AGS performance during the treatment of wastewater containing pharmaceuticals, a frequent scenario in WWTP.

[1]  P. Castro,et al.  Chiral pharmaceuticals in the environment , 2012, Environmental Chemistry Letters.

[2]  Abdeltif Amrane,et al.  Removal of antibiotics by an integrated process coupling photocatalysis and biological treatment - Case of tetracycline and tylosin , 2011 .

[3]  Damià Barceló,et al.  Contribution of hospital effluents to the load of pharmaceuticals in urban wastewaters: identification of ecologically relevant pharmaceuticals. , 2013, The Science of the total environment.

[4]  Liwei Sun,et al.  Toxicity and enantiospecific differences of two β‐blockers, propranolol and metoprolol, in the embryos and larvae of zebrafish (Danio rerio) , 2014, Environmental toxicology.

[5]  Dawen Gao,et al.  Aerobic granular sludge: characterization, mechanism of granulation and application to wastewater treatment , 2011, Critical reviews in biotechnology.

[6]  Xiurong Chen,et al.  Exploring the relationship between the EPS property and the toxicity of sludge for treating 4-chlorophenol synthetic wastewater in a sequencing batch reactor , 2016 .

[7]  B. Kasprzyk-Hordern,et al.  A review on emerging contaminants in wastewaters and the environment: current knowledge, understudied areas and recommendations for future monitoring. , 2015, Water research.

[8]  P. Castro,et al.  Enantioselective biodegradation of pharmaceuticals, alprenolol and propranolol, by an activated sludge inoculum. , 2013, Ecotoxicology and environmental safety.

[9]  C. Helbing,et al.  Evaluating the treatment of a synthetic wastewater containing a pharmaceutical and personal care product chemical cocktail: compound removal efficiency and effects on juvenile rainbow trout. , 2014, Water research.

[10]  Charles S Wong,et al.  Stereoisomer analysis of wastewater-derived beta-blockers, selective serotonin re-uptake inhibitors, and salbutamol by high-performance liquid chromatography-tandem mass spectrometry. , 2007, Journal of chromatography. A.

[11]  H. Horn,et al.  Aerobic Sludge Granulation in a Full-Scale Sequencing Batch Reactor , 2014, BioMed research international.

[12]  C. M. Hooijmans,et al.  Temperature effects on glycogen accumulating organisms. , 2009, Water research.

[13]  M. V. van Loosdrecht,et al.  Performance of aerobic granular sludge in a sequencing batch bioreactor exposed to ofloxacin, norfloxacin and ciprofloxacin. , 2014, Water research.

[14]  Geoffrey Michael Gadd,et al.  Biosorption: current perspectives on concept, definition and application. , 2014, Bioresource technology.

[15]  Xinhua Wang,et al.  Changes of the reactor performance and the properties of granular sludge under tetracycline (TC) stress. , 2013, Bioresource technology.

[16]  J. Tay,et al.  Aerobic granular sludge: recent advances. , 2008, Biotechnology advances.

[17]  C. Delerue-Matos,et al.  Enantiomeric fraction evaluation of pharmaceuticals in environmental matrices by liquid chromatography-tandem mass spectrometry. , 2014, Journal of chromatography. A.

[18]  M. V. van Loosdrecht,et al.  Formation of aerobic granules and conversion processes in an aerobic granular sludge reactor at moderate and low temperatures. , 2005, Water research.

[19]  S. Cravo,et al.  Fluoroquinolones biosorption onto microbial biomass: activated sludge and aerobic granular sludge , 2016 .

[20]  A. Durán,et al.  Mineralization of wastewater from the pharmaceutical industry containing chloride ions by UV photolysis of H2O2/Fe(II) and ultrasonic irradiation. , 2014, Journal of environmental management.

[21]  M. V. van Loosdrecht,et al.  Removal of fluoxetine and its effects in the performance of an aerobic granular sludge sequential batch reactor. , 2015, Journal of hazardous materials.

[22]  J. Tay,et al.  Microbial adaptation to biodegradation of tert-butyl alcohol in a sequencing batch reactor. , 2005, Journal of biotechnology.

[23]  P. Castro,et al.  Integrated liquid chromatography method in enantioselective studies: Biodegradation of ofloxacin by an activated sludge consortium. , 2016, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[24]  T. Wu,et al.  A case study of antibiotic wastewater treatment by using a membrane biological reactor system , 2015 .

[25]  I. Moreira,et al.  Enantioselective quantification of fluoxetine and norfluoxetine by HPLC in wastewater effluents. , 2014, Chemosphere.

[26]  M. Loosdrecht,et al.  Selection of slow growing organisms as a means for improving aerobic granular sludge stability , 2004 .

[27]  Zhong-lin Chen,et al.  Remediation of pharmaceuticals and personal care products using an aerobic granular sludge sequencing bioreactor and microbial community profiling using Solexa sequencing technology analysis. , 2015, Bioresource technology.

[28]  Aaron Marc Saunders,et al.  Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source. , 2006, Microbiology.

[29]  L. Shu,et al.  Characterization of the extracellular polymeric substances and microbial community of aerobic granulation sludge exposed to cefalexin , 2015 .

[30]  S. Okabe,et al.  Effect of oxygen concentration on nitrification and denitrification in single activated sludge flocs , 2003, Biotechnology and bioengineering.

[31]  A. R. Ribeiro,et al.  Enantioseparation of chiral pharmaceuticals in biomedical and environmental analyses by liquid chromatography: an overview. , 2014, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[32]  Klaus Kümmerer,et al.  The presence of pharmaceuticals in the environment due to human use--present knowledge and future challenges. , 2009, Journal of environmental management.

[33]  Zhiguo Yuan,et al.  Breakage and growth towards a stable aerobic granule size during the treatment of wastewater. , 2013, Water research.

[34]  S. M. Shafie,et al.  Enantiomeric fraction as an indicator of pharmaceutical biotransformation during wastewater treatment and in the environment – a review , 2010, Environmental technology.

[35]  Damià Barceló,et al.  Applications of LC-MS to quantitation and evaluation of the environmental fate of chiral drugs and their metabolites , 2008 .

[36]  B. Kasprzyk-Hordern,et al.  Enantiomeric analysis of drugs of abuse in wastewater by chiral liquid chromatography coupled with tandem mass spectrometry. , 2010, Journal of chromatography. A.

[37]  B. Kasprzyk-Hordern.  Pharmacologically active compounds in the environment and their chirality. , 2010, Chemical Society reviews.

[38]  L. M. M. de Bruin,et al.  Advancements in the application of aerobic granular biomass technology for sustainable treatment of wastewater , 2013 .

[39]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[40]  Á. Ríos,et al.  Use of toxicity assays for enantiomeric discrimination of pharmaceutical substances. , 2009, Chirality.

[41]  M C M van Loosdrecht,et al.  Selective sludge removal in a segregated aerobic granular biomass system as a strategy to control PAO-GAO competition at high temperatures. , 2011, Water research.

[42]  T. Oliveira,et al.  Characterization of Pharmaceuticals and Personal Care products in hospital effluent and waste water influent/effluent by direct-injection LC-MS-MS. , 2015, The Science of the total environment.